Characterization of the Phytochemicals and Antioxidant Properties of Extracts From Wrightia Tinctoria R.Br.
Main Article Content
Abstract
In the present investigation, phytochemical screening of different extracts of Wrightia tinctoria has been tested by using standard procedures. The findings showed the presence of alkaloids, flavonoids, phenols, steroids, terpenoids and glucosides. Among the four solvents tested (aqueous, ethanol, N-hexane and ethyl acetate) ethanol extract of leaf showed the best result. The free radical scavenging activity of both the extract was measured by using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The aqueous and ethanol extracts of Wrightia tinctoria leaf shows slightly different antioxidant activity. The remarkable activity showed by the plant is due to the synergic effect of active compounds present in it.Characterization of phytochemicals was done by using UV-Visible spectrophotometry, FTIR analysis and Thin layer chromatography
Downloads
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Brown R, Wrightia tinctoria R. Br., Mem Wern Nat Hist Soc., 1810; 1: 74-75
Khyade MS AND Vaikos NP: Comparitive phytochemical and antibacterial studies on the bark of Wrightia tinctoria and Wrightia arborea. Int J. Pharm Bio Sci. 2011; 2: 176-181.
Singh B, Gupta V, Bansal P, Singh R and Kumar D: Pharmacological potential of plant used as aphrodisiacs. Int J Pharm Sci Rev and Res., 2010; 5(1): 104-113.
Nagarani B, Debnath S, Kumar SC, Bhattacharjee C and Kumar GG: A review : herbs used as anticancer agents. IRJP.2011; 2(1): 20-24.
Harbone JB: Phytochemical methods, A guide to modern techniques of plant analysis, London: Chapman and Hall Ltd.,1973.
Kannan P, Shanmugavadivu B, Petchiammal C and Hopper W : In vitro antimicrobial activity of Wrightia tinctoria leaf extract against skin microorganisms. Acta Botanica Hungarica.2006; 48(3-4):323-329.
Shalini S and Sampath Kumar P: Phytochemical screening and antimicrobial activity of plant extracts for disease management. Int J Curr Sci.2012; 209-218.
Yogesh M and Mohan JSS: Screening of plants for their potential antibacterial activity against Staphylococcus and Salmonella sp. Natural Product Radiance 2007; 6(4): 301-305.
A Yadav, Bgardwaj R ans Sharma RA: Int. j. Chem Tech. Res, 2011; 3:1054.
Zahin M , Ahmad L, Aqil F.2017- Antioxident and antimutagenic potential of Psidium guajava leaf extracts. Drug and chemical toxicology 40(2), 146-153.
Akbay P, Basaran AA, Undeger U, In vitro immune modulatoy activity of flavonoid glycosides from Utricadiocia L, Phytother Res ., 17,2003,34-37
Reddi CS, Reddi EUB and Reddi MS. A novel mechanism of pollination in Wrightia tinctoria R.Br. Curr Sci., 1979; 48(16): 746-747.
Rao PS, Maheswar Rao G and Satyanarayana VVV. Rooting of stem cuttings of Wrightia tinctoria (Roxb.) R.Br: An important medicinal plant. Indian Forester., 1999; 125(4): 427-428.
Suresh DR, Hegde GV and Ganesh KB, Vegetative propagation of Wrightia tinctoria R.Br. - An important multipurpose tree. Indian Forester, 2004; 130(8): 950-952.
Sarada S, Nair GS and Reghunath BR. Quantification of medicinally valuable weeds in oil palm plantations of Kerala. Journal of Tropical Agriculture, 2002; 40: 19-26.
Gill BS, Singhal VK, and Bedi YS and Bir SS. Cytological evolution in the woody taxa of Pachmarhi Hills. J Cytol Genet., 1990; 25: 308–320.
Khan K, Sharma SK, Ramarao S and Purohit SD, Karyomorphological Studies in Wrightia tomentosa and W. tinctoria, Multipurpose Tree Species of Aravallis in South East Rajasthan. Cytologia, 2011; 76(2): 125-128.
Orwa C, Mutua A, Kindt R, Jamnadass R and Anthony S, Agroforestree Database: a tree reference and selection guide version 4.0 (World Agroforestry Centre, Kenya), 2009.
Sethi PD. Separation of alkaloidal constituents of Wrightia tinctoria by TLC. Planta med., 1970; 18(1): 26-29.
Reddy YSR, Venkatesh S, Ravichandran T, Murugan V and Suresh B. Antinociceptive activity of Wrightia tinctoria bark. Fitoterapia, 2002; 73: 421-423.
Bigoniya P and Rana AC. Absence of central activity in Wrightia tinctoria bark ethanolic extract. J Pharm Neg Res., 2010; 1(2): 51-54.
Tomar R, Kumar R and Jagannadham MV. A stable serine protease, Wrightin, from the latex of the plant Wrightia tinctoria (Roxb.) R. Br.: purification and biochemical properties. J Agri Food Chem., 2008; 56: 1479-1487.
Kannan P, Shanmugavadivu B, Petchiammal C and Hopper W. In vitro antimicrobial activity of Wrightia tinctoria leaf extracts against skin microorganisms. Acta botanica Hungarica, 2006; 48(3-4): 323-329.
Yogesh M and Mohan JSS. Screening of plants for their potential antibacterial activity against Staphylococcus and Salmonella spp. Natural Product Radiance., 2007; 6(4): 301305.
Ranjani S, Deepa M, Kalaivani K and Sheela P. Antibacterial and antifungal screening of ethanol leaf extract of Wrightia tinctoria against some pathogenic microorganisms. Drug Invention Today, 2012; 4(5): 365-367.
Brown R, Wrightia tinctoria R. Br., Mem Wern Nat Hist Soc., 1810; 1: 74-75.
Nasir E, Ali SI (Eds), Fl. Pakistan, Univ. of Karachi, Karachi, 1980-2005.
Ngan PT, A revision of the genus Wrightia (Apocyanaceae). Ann Miss Bot Gard., 1965; 52: 114–175.
Joshi SG, Medicinal plants (Oxford & IBH publishing Co. Pvt. Ltd., New Delhi), 2000; 51-52. 5. “The Plant List Version 1.1”. The plantlist.org, 2013. Retrieved 21 April 2016
Ganesan S. Traditional oral care medicinal plants survey of Tamilnadu. Natural Product Radiance, 2008; 7(20): 166-172.
Rao JK, Seetharami Reddi TVV and Kumar OA. Ethanobotany of stem bark of certain plants of Visakha patnam district, Andhra Pradesh. Current Botany, 2011; 2(5): 01-06.
Singh B, Gupta V, Bansal P, Singh R and Kumar D. Pharmacological potential of plant used as aphrodisiacs. Int J Pharm Sci Rev and Res., 2010; 5(1): 104-113.
Nagarani B, Debnath S, Kumar S C, Bhattacharjee C and Kumar GG. A review: herbs used as anticancer agents. IRJP, 2011; 2(1): 20-24.
Lin CC, Hsu YA, Lin TC. Effects of punicalagin on carrageenan- induced inflammation in rats, The Amr J Chin Med 1999; 27:371-376
Desmarchelier C, Bermudez MJN, Coussio J, Ciccia G, Boveris A. Antioxidant and prooxidant activities in aqueous extract of Argentine plants. Int J Pharmacogn. 1997;35:116–20.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199–200.
Zahin M, Ahmad I, Aqil F. 2017 – Antioxidant and antimutagenic potential of Psidium guajava leaf extracts. Drug and Chemical Toxicology 40(2),146–153.
Akbay P, Basaran AA, Undeger U, Basaran N, In vitro immune modulatory activity of flavonoid glycosides from Utricadioica L, Phytother Res., 17, 2003, 34-37.
Shamsa F, MengchengT, Jianming W. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci 2008;32:17-20.
Shahverdi A R, Abdolpour F, Monsef-Esfahani H R, Farsam H A. TLC bioautographic assay for the detection of nitrofurantoin resistance reversal compound. J Chromatogr B. 2007;850:528–530.
Das Talukdar, M. Dutta Choudhury, M.Chakraborty, B.K. Dutta, Phytochemical screening and TLC profiling of plant extracts of Cyatheagigantea (Wall. Ex. Hook.) Haltt. And Cyathea brunoniana. Wall. ex. Hook.(Cl. & Bak.), Biological and Environmental Science, 5(1), 2010; 70-74.