Phytochemical Standardization Of Aegle Marmelos Leaves Extract By Using High Performance Thin Layer Chromatography Fingerprinting (Hptlc)
Main Article Content
Abstract
Objective: Aegle marmelos is well known for its therapeutic properties in the Indian traditional medicinal system. The presence of important bioactive compounds in plants makes them pharmacologically valuable. Therefore, in the present study, the high-performance thin layer chromatography (HPTLC) analysis of hydroalcoholic extract of Aegle marmelos was performed for its phytochemical profiling.
Methods: Preliminary phytochemical screening was done. HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, Reprostar 3 and WIN CATS were used. The HPTLC densitometric analysis of the hydroalcoholic extract of Aegle marmelos was carried out using CAMAG HPTLC system, and the results were obtained in the form of chromatograms (scanned at the wavelength of 254 nm and 366 nm) representing several peaks.
Results: Preliminary phytochemical screening of the extract showed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, terpenoids, phenolic compounds, proteins, fats and oils. TLC resulted in identification of three spots found in the hydroalcoholic extract. The phytochemical profile of the plant was determined and presented in the tables showing the total number of peaks, peak heights, peak area, percent area, and Rf values. HPTLC finger printing of hydroalcoholic extract of Aegle marmelos under white light reveal presence of five components 0.04, 0.03, 0.29, 0.42, 0.49. Component number 2,3 and 4 at Rf 0.03, 0.29 and 0.42 showed maximum concentration.
Conclusion: The study concluded that hydroalcoholic extract of Aegle marmelos contains a rich variety of phytochemicals which might be accountable for its therapeutic value and thus justifies its traditional use in India.
Keywords: Aegle marmelos, HPTLC, Alkaloids, hydroalcoholic extract
Objective: Aegle marmelos is well known for its therapeutic properties in the Indian traditional medicinal system. The presence of important bioactive compounds in plants makes them pharmacologically valuable. Therefore, in the present study, the high-performance thin layer chromatography (HPTLC) analysis of hydroalcoholic extract of Aegle marmelos was performed for its phytochemical profiling.
Methods: Preliminary phytochemical screening was done. HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, Reprostar 3 and WIN CATS were used. The HPTLC densitometric analysis of the hydroalcoholic extract of Aegle marmelos was carried out using CAMAG HPTLC system, and the results were obtained in the form of chromatograms (scanned at the wavelength of 254 nm and 366 nm) representing several peaks.
Results: Preliminary phytochemical screening of the extract showed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, terpenoids, phenolic compounds, proteins, fats and oils. TLC resulted in identification of three spots found in the hydroalcoholic extract. The phytochemical profile of the plant was determined and presented in the tables showing the total number of peaks, peak heights, peak area, percent area, and Rf values. HPTLC finger printing of hydroalcoholic extract of Aegle marmelos under white light reveal presence of five components 0.04, 0.03, 0.29, 0.42, 0.49. Component number 2,3 and 4 at Rf 0.03, 0.29 and 0.42 showed maximum concentration.
Conclusion: The study concluded that hydroalcoholic extract of Aegle marmelos contains a rich variety of phytochemicals which might be accountable for its therapeutic value and thus justifies its traditional use in India.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Sisodiya D, Shrivastava P (2018) Antimicrobial activity of Euphorbia thymifolia (L.) and Manilkara hexandra (Roxb.). Int J Curr Adv Res 7(2):9660–9663
Latha SP, Kannabiran K (2006) Antimicrobial activity and phytochemicals of Solanum trilobatum Linn. Afr J Biotechnol 5:2402–2404
Sisodiya D, Shrivastava P (2018) Phytochemical screening, thin layer chromatography and quantitative estimation of bioactive constituents in aqueous extract of Manilkara hexandra (Roxb.) dubard. Int J Recent Sci Res9(1):23083–23086
Cooper LN, Blais BS (2004) Theory of cortical plasticity. World scientific publishing, Singapore
N. Malliga Elangovan1, M.S. Dhanarajan, I. Elangovan (2015) Preliminary Phytochemical Screening and HPTLC Fingerprinting Profile of Leaf Extracts of Moringa oleifera and Phyllanthus emblica. International Research Journal of Pharmaceutical and Biosciences (IRJPBS) 2 (2) 32- 40
Savita L., Singh A., Singh A. (2021) Aegle marmelos (Bael): A Systematic Review Journal of Drug Delivery & Therapeutics.; 11(3-s):131-136
Jagannadha Rao G H.. and Lakshmi P. (2012) Evaluation of Antidiarrhoeal activity of extract from leaves of Aegle marmelos Journal of Applied Pharmaceutical Science 02 (02); 75-78
Tilburt JC, Kaptchuk TJ (2008) Herbal medicine research and global health: an ethical analysis. Bull World Health Organ 86:594–599
Sisodiya D, Shrivastava P (2017) Qualitative and quantitative estimation of bioactive compounds of Euphorbia thymifolia L. Asian J Pharm Edu Res 6(3):34–43
Kokate CK. Practical Pharmacognosy. Vallabh Prakashan, New Delhi. 2005; 4th Edition: 106-109.
Khandelwal KR. Practical Pharmacognosy. Nirali Prakashan, Pune. 2007; 16th Edition: 149-156.
Tiwari, P., Kumar, B., Kaur, M., Kaur, G. and Kaur, H., 2011. Phytochemical screening and extraction: a review. Internationale pharmaceutica sciencia, (1), pp.98-106.
Singh GK, Bhandari A. Text book of Pharmacognosy. CBS Publisher & Distributor, New Delhi. 2005; 1st Edition: 39-62.
Gupta RC, Bhargava S. Practical Biochemistry. CBS Publisher & Distributor New Delhi. 2006; 4th Edition: 10-44.
Lehman, J. W. The student's lab companion: laboratory techniques for organic chemistry: standard scale and microscale. Pearson College Div, (2008).
Pavia, D. L., Lampman, G. M., Kriz, G. S., & Engel, R. G. Microscale and Macroscale Techniques. Thomson Wadsworth, (2006).
Pannkuk, E. L., Risch, T. S., Savary, B. J. (2013) Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry. J. Vis. Exp. (79), e50757.
Kagan, I. A., Flythe, M. D. (2014) Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds. J. Vis. Exp. (85), e51411.
Egon Stahl, TLC- A laboratory handbook, 2nd edition, international student edition, New York, 346-349, 421-461.
Wagner H. and Bladt S, Plant Drug Analysis -and A Thin Layer Chromatographic Atlas, Springer- Verlag Berlin Heidelberg, 1996.
Harborne, J.B., Phytochemical methods, 3rd edition, Chapman and Hall, London, 1998.
Fatemeh Fathiazada, Abbas Delazara, (2006) Extraction of Flavonoids and Quantification of Rutin from waste Tobacco Leaves, Iranian Journal of Pharmaceutical Research 3: 222-227.