Phytochemical Screening, Invitro Antioxidant and In-vivo Antidiabetic Activity of Methanolic Extract of Leaves of Celastrus paniculatus
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Abstract
Celastrus paniculatus Willd, commonly known as Staff tree, is a plant species from the Celastraceae family. It contains various active constituents, including celapanigin, malkangunin, celapanin, and zeylasteral, which contribute to its medicinal properties. The objective of this study was to evaluate the qualitative and quantitative phytochemical analysis, in vitro antioxidant activities, and in vivo anti-diabetic potentials of the methanolic extract of C. paniculatus leaves using a streptozotocin-induced rat model. The in vitro antioxidant activity of the methanolic extract was assessed using DPPH and ABTS radical cation decolorization assay methods. Rats were induced with diabetes using streptozotocin and treated with glibenclamide as the standard medication. Body weight and blood sugar levels were measured throughout the study. Phytochemical analysis revealed the presence of alkaloids, glycosides, proteins and amino acids, flavonoids, tannins and phenolic compounds, saponins, triterpenoids, and steroids in the leaves. The total phenolic content of the methanolic extract was 32.33 mg/g, followed by flavonoids at 11.66 mg/g. Oral treatment with the methanolic extract of C. paniculatus at doses of 200 and 400 mg/kg significantly reduced blood glucose levels in diabetic rats compared to control rats (p < 0.001) and increased body weight. The chemical constituents of the plant extract may have potential in preventing diabetic complications and could serve as an alternative in the current range of antidiabetic drugs. Further research is recommended to validate the use of this plant as an antidiabetic agent
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