Qualitative and Quantitative estimation of Phytoconstituents and Antimycobacterial property of ethanolic extract of Cassia fistula leaf against H37RV test organism

Abstract

Cassia fistula belongs to the family Caesalpinaceae commonly known as “Golden shower tree” has been used in different traditional system of medicines for various ailments since ancient times. Cassia fistula grows throughout in Bangladesh and in many other Asian countries such as India, China, Hong Kong, Philippines, Malaysia, Indonesia, and Thailand. Sequential extraction of leaves of Cassia fistula was carried out by using different solvents like petroleum ether, chloroform, ethanol, methanol and water in Soxhlet apparatus. Ethanolic extract was investigated for qualitative and quantitative phytochemical studies. Ethanolic extract was also analysed for its antimycobacterial property. Results of the study showed highest yield of the leaf extract in ethanol. Phytochemical studies revealed the presence of secondary metabolites like carbohydrate, proteins, amino acids, steroids, saponins, alkaloids, tannins, phenols and flavanoids. Ethanolic leaf extract showed good inhibitory activity against H₃₇RV test organism. The minimum inhibitory concentration was found to be 2.5μg/ml. Furthermore, the ethanol extract was subjected to LCMS, TLC and HPTLC fingerprinting. Results of LCMS, TLC and HPTLC showed the presence of alkaloids, flavonoids and phenols.