Antioxidant And Anti-Mycotoxin Activities, And Cytotoxicity Properties In Vitro Of Propolis Extracts

Abstract

Abstract: The present study aims to determine the antioxidant activity and cytotoxic activity of propolis and evaluate the anti-mycotoxin activity of different propolis extracts. The three extracts of propolis (ethyl acetate, ethanol (75%), and water) were subjected to testing of the antioxidant potential, and total phenolic and flavonoid contents. determination of the cytotoxicity activity by standard cell culture method against colon and liver and the ability of propolis extracts to control aflatoxin (AF) production. The results revealed that the highest value of antioxidant activities was found for propolis ethyl acetate extract with an average % inhibition of 63.42%. the highest amount of propolis's total phenolic and flavonoid contents using ethyl acetate extract were 149.5 µg gallic acid equivalent/mg extract and 218.1 µg quercetin equivalent/mg extract, respectively. The three propolis extracts affect liver and colon human cell cancer. Ethyl acetate extract of propolis showed the lowest IC₅₀ with the highest anti-cancer activity on the liver cancer cell line. In comparison, water extract showed the lowest IC₅₀ with the greatest anticancer activity on the colon cancer cell line (50 and 54 µg/ml, respectively). Using different concentrations of propolis to reduce AF levels led to the complete disappearance of AF production at 100, 200, and 400 mg/ml concentrations of Ethyl acetate extract against AF production. In conclusion, these obtained findings indicated that propolis extracts exhibited substantial antioxidant and anti-cancer activities.