Phytometabolite Profiling Of Combretum Indicum (L.) Defilipps And Its Characterization Studies

Main Article Content

Sidharth. K
K. Narayanasamy
Sruthy Mohan
K. Baskaran
Nirmala Devi. N
R. Ragunathan

Abstract

Combretum indicum (L.) DeFilipps., also known as Quisqualis indica, belongs to the combretaceae family. Literature suggests the hardy nature and predominant presence of medically important phytometabolites within these plants. This study aimed to identify the phytochemical constituents of the Combretum indicum flower extracts. Flower extracts were prepared using Aqueous, ethanol, n-Hexane and petroleum ether as the solvents of choice. Qualitative phytochemical analysis indicated the presence of phytometabolites including alkaloids, flavonoids, terpenoids, phenols, quinines, steroids, saponins etc. Among these solvents ethanolic flower extract showed better results. The quantitative analysis of flower extract indicated the presence good quantity of flavonoids and phenols respectively. Anti-oxidant activity was tested using DPPH scavenging assay. Characterization of phytochemicals was done by using UV-Visible spectrophotometry, FTIR analysis and thin layer chromatography.

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How to Cite
Sidharth. K, K. Narayanasamy, Sruthy Mohan, K. Baskaran, Nirmala Devi. N, & R. Ragunathan. (2024). Phytometabolite Profiling Of Combretum Indicum (L.) Defilipps And Its Characterization Studies. Journal of Advanced Zoology, 45(3), 824–830. https://doi.org/10.53555/jaz.v45i3.4493
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Articles
Author Biographies

Sidharth. K

Department of Biochemistry, Sree Narayana Guru College, K.G. Chavadi, Coimbatore.

K. Narayanasamy

Department of Biochemistry, School of Life sciences, Nehru Arts and Science College, Coimbatore.

Sruthy Mohan

Department of Biochemistry, School of Life sciences, Nehru Arts and Science College, Coimbatore.

K. Baskaran

Department of Biochemistry, Sree Narayana Guru College, K.G. Chavadi, Coimbatore.

Nirmala Devi. N

Department of Biochemistry, Sree Narayana Guru College, K.G. Chavadi, Coimbatore.

R. Ragunathan

Director of Center for Bioscience and Nano Science research, Echanari, Coimbatore.

References

Wink, M. Schimmer, O. Modes of action of defensive secondary metabolites. Annu. Plant Rev, 2018, 2, 18-137.

Igwe CU, Nwagou LA, et al., Assessment of the hepatic effects, phytochemical and proximate compositions of Phyllanthus amarus. Afr J Biotech, 2007, 6, 728-731.

Ojiako OA, Nwanjo HU. Biochemical studies of the effects of the aqueous extract of Nigerian garlic on lipid profile and atherogenic risk predicter indices. Aust J Basic Applied Sci, 2009, 3, 2861-2865.

Rao, S.R. Ravishankar, G.A. Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol. Adv, 2002, 2, 101-153.

Wink, M. Introduction: Biochemistry, role and biotechnology of secondary metabolites. Annu. Plant Rev, 2018, 3,1-17.

Lee, Y.H. Wang, C.M. Liu, P.Y. et al., volatile oils of nepeta tenuifolia as an alternative medicine against multidrug-resistant pathogenic microbes Can. J. Infect. Dis. Med. Microbiol, 2018, 1.

Mtunzi, F.M Ejidike, I.P et al., Solvent-solvent fractionations of Combretum erythrophyllum (Burch) leaf extract: studies of their antibacterial, antifungal, antioxidant and cytotoxicity potentials. Asian pac.j.Trop. Med, 2017, 10, 670-679.

Alfei, S. Caviglia, D. Penco, S. Zuccari, G. Gosetti, F. 4-Hydroxybenzoic Acid as an Antiviral Product from Alkaline Autoxidation of Catechinic Acid: A Fact to Be Reviewed. Plants , 2022, 11, 1822.

Eloff, J.N. Katerere, D.R. McGaw, L.J. The biological activity and chemistry of the Southern African Combretaceae. J. Ethnopharmacol, 2008, 119, 686–699.

Lima, G. Sales, P.; Filho, M. Jesus, N. Falcão, H. Barbosa-Filho, J. Cabral, A. Souto, A. Tavares, J. Batista, L. Bioactivities of the genus Combretum (Combretaceae): A review. Molecules, 2012, 17, 9142–9206.

T. L. Do. Vietnamese medicinal plants and herbs, Medical Publishing House, 2004, 156- 157.

P.C. Pan, S.D. Fang, C.C. Tsai. The chemical constituents of Shihchuntze, Quisqualis indicaL. II. Structure of Quisqualis acid, Sci.Sin,1976,19(5), 691-701

M. Kumar,N.R.S. Govt. Antibacterial activity of Combretum indicum(L.) DeFilipps flower extracts against gram-positive and gram-negative human pathogenic bacteria, World. J. Pharm. Pharm. Sci, 2015, 4(10), 1288-1297

S.H. Tadros, H.H. Eid, C.G.Michel, A.A.Sleem.Phytochemical and biological study of Quisqualis indicaL. grown in Egypt, Egypt. J. Biomed. Sci, 2004, 15, 414-434

Kumari et al., Analgesic activity of Quisqualis indica, Pharm.Chem. J, 2017, 4(1), 1-8

Wetwitayaklung, P. et al., Kinetics of acetylcholinesterase inhibition of Quisqualis indica Linn. flower extract, SUST.J, 2007, 1(2), 20-28

E.R. Abd, Ahmed A.A., Abd E.A., Ibrahim M., Refahy L.A., El-Shazly M.A. Total phenolic content, cytotoxic and antioxidant activities of Quisqualis indica (Linn.) growing in Egypt, Der Pharma Chem, 2016, 8, 53-59

Wijerathne, C.U, Park, H.S. Jeong, H.Y. J. W. Song, O.S. Moon, Y. W. Seo, Y. S. Won, H.Y. Son, J.H. Lim, S.H. Yeon, et al. Quisqualis indica improves benign prostatic hyperplasia by regulating prostate cell proliferation and apoptosis, Biol.Pharm. Bull, 2017,40(12), 2125-2133

Sahu, J. Patel, P.K. Dubey B. Hypolipidemic effect of Quisqualis indica (Linn) aerial parts on passive smoking & cholesterol diet fed animals, J.Pharm.Res, 2012, 5(9), 4671-4675.

Nair, G.A. Joshua, C.P. Nair A.G.R. Flavonoids of the leaves and flowers of Quisqualis indica Linn, Indian J. Chem. Sect. B, 1979, 18B (3), 291-292

Lin, T.C. Ma, Y. T. Wu, J. Hsu F. L. Tannins and related compounds from Quisqualis indica, J. Chin. Chem. Soc,1997, 44(2), 151-155

Efferth, T. Kahl, S. Paulus, K. Adams, M. Rauh, R. Bouchet, H. Hao, X. Kaina B., Bauer. R. Phytochemistry and pharmacogenomics of natural products derived from traditional Chinese medicine and Chinese Materia medica with activity against tumor cells, Mol. Cancer. Ther, 2008, 7(1), 152-161

Jahan, F.N. Rahman, M.S. Rahman, M.M. Gibbons, S. Masud, M.M. Sadhu, S.K. Hossain, M. Hasan, C. M. Rashid. M.A., Di phenylpropanoids from Quisqualis Indica Linn. and their anti-staphylococcal activity, Lat.Am.J.Pharm, 2009, 28(2), 279-283.

Excitotoxic cell death and delayed rescue in human neurons derived from NT2 cells, M Munir, L Lu and P Mcgonigl, Journal of Neuroscience, 1997, 15, 7847–7860

Glutamate cytotoxicity in a neuronal cell line is blocked by membrane depolarization. T. H. Murphy, R. L. Schnaar, J. T. Coyle and A. Sastre. Brain Research, 1988, 460 (13),155–160

Zahin M, Ahmad I, Aqil F. Antioxidant and antimutagenic potential of Psidium guajava leaf extracts. Drug and Chemical Toxicology , 2017, 40(2), 146–153.

Akbay P, Basaran AA, Undeger U, Basaran N, in vitro immune modulatory activity of flavonoid glycosides from Utricadioica L, Phytother Res, 2003, 17, 34-37.

Desmarchelier C, Bermudez MJN, Coussio J, Ciccia G, Boveris A. Antioxidant and prooxidant activities in aqueous extract of Argentine plants. Int J Pharmacogn.1997,35:116–20.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature,1958; 181:1199–200.

Shahverdi A R, Abdolpour F, Monsef-Esfahani H R, Farsam H A. TLC bioautographic assay for the detection of nitrofurantoin resistance reversal compound. J Chromatogr B, 2007; 850:528–530.

Das Talukdar, A. Dutta Choudhury, M. Chakraborty, M. Dutta, B.K. Phytochemical screening and TLC profiling of plant extracts of Cyatheagigantea (Wall. Ex. Hook.) Haltt. And Cyathea brunoniana. Wall. ex. Hook. (Cl. & Bak.), Biological and Environmental Science, 2010, 5(1); 70-74.