Molecular Identification, Antioxidant And Anticancer Potential Of Endophytic Fungi From Moringa Oleifera
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Abstract
Endophytic fungi inhabit apparently healthy plant tissues and are prevalent in terrestrial plants, especially root tissues, which harbour a wide assemblage of fungal endophytes. Therefore, this study focused on the isolation and characterisation of endophytic fungi from the leaves of M. oleifera. The endophytic fungi were initially sorted according to morphological characteristics and identified using the sequences of the Internal Transcribed Spacer (ITS) having 18-s RNA regions of other fungi. The most common fungal isolates were species of the genus Acremonium strictum AF2B-3B (AccessionNumber:CU737841.2). This study showed that the leaves of M. oleifera harbour a diverse group of endophytic fungi. The most potent fungal isolate, antioxidant, and anticancer activity against MCF-7, 3T3 cancer cell lines. The findings revealed that leaves of M. oleifera has the capacity to host a wide variety of fungal endophytes and that secondary metabolites from the endophytic fungus may be a source of alternative naturally occurring antioxidant, and cytotoxic compounds
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