A Comparative In Vitro Antioxidant Potential Of Various Extracts From Aerial And Underground Parts Of Dalbergia Sissoo.
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Abstract
Objective: To evaluate antioxidant and radical scavenging activities of aerial and underground parts of Dalbergia sissoo extracts.
Methods: Shed dried and powdered plant parts were initially extracted in methanol and subsequently partitioned in n-hexane, chloroform, ethyl acetate and 1-butanol successively. Antioxidant and radical scavenging potential of methanol extracts and fractions of each parts were evaluated using Total phenolic content (TPC) and Total flavonoid content (TFC), 2,2- diphenyl-1-picrylhydrazyl and 2,2’ –azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation radical scavenging, reducing power (potassium ferricyanide-trichloroacetic acid system), ferric ion reducing antioxidant potential, lipid peroxidation inhibition activity and total antioxidant activity assays.
Results: TPC and TFC values for methanol extracts and various fractions ranged from 0.23- 4mg/L gallic acid equivalents and from 30-545 mg/L rutin equivalent respectively, Methanol extracts and all fractions of roots and aerial parts showed higher TPC and TFC values. Methanol extracts and aqueous fractions of roots and aerial parts and n-butanol fractions of root showed lower EC50 values for 2, 2- diphenyl-1-picrylhydrazyl scavenging than the other plant extracts. The 2, 2’ –azinobis , radical scavenging, total antioxidant potential and ferric ion reducing antioxidant potential values confirmed the presence of potent antioxidant potential values confirmed the presence of potent antioxidant principles in the methanol extract of roots. All the extracts of the roots showed high antioxidant and radical scavenging activities.
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