“Unveiling Phoenix sylvestris: Phytochemical Insights, Antioxidant Potential and Antiproliferative Impact on HT29 Cells”
DOI:
https://doi.org/10.53555/jaz.v45i6.4825Keywords:
Colon cancer, Molecular docking, antioxidants, Phoenix sylvestris, Medicinal plantsAbstract
Phoenix sylvestris, commonly known as the wild date palm, holds immense potential in traditional medicine due to its diverse phytochemical composition. Leaf identification plays a crucial role in botanical research, ecological studies, and biodiversity conservation. Phoenix sylvestris, commonly known as the wild date palm or silver date palm, is an economically and ecologically significant palm species. Traditional methods of identifying plant species based on morphological characteristics can be challenging, especially when dealing with closely related species or in cases of morphological variation. In this study, we present a DNA-based approach for the identification of Phoenix sylvestris leaves through DNA extraction, amplification of specific genetic markers, and sequencing. Leaf samples were collected from various geographical locations to encompass genetic diversity. DNA was extracted using a modified CTAB method, and the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA was amplified using polymerase chain reaction (PCR). Sanger sequencing was performed to obtain DNA sequences, which were then compared to reference sequences in public databases for species identification. The results demonstrate the efficacy of this approach in accurately identifying Phoenix sylvestris leaves, even in cases where morphological characteristics may be ambiguous or variable. This DNA-based method provides a reliable tool for rapid and accurate identification of Phoenix sylvestris, contributing to its conservation and management efforts, as well as facilitating research on its ecology, distribution, and evolutionary history. This study presents a multifaceted analysis of P. sylvestris extract, encompassing phytochemical characterization, antioxidant, antimicrobial, and anticancer evaluations, along with molecular docking simulations. The phytochemical analysis revealed the presence of various bioactive compounds such as phenolics and flavonoids contributing to its medicinal properties. Assessment of antioxidant activity through DPPH assay demonstrated significant radical scavenging potential, indicating its potential therapeutic application in oxidative stress-related disorders. Moreover, the antimicrobial evaluation against a panel of pathogenic microorganisms highlighted the extract's efficacy in inhibiting microbial growth, suggesting its utility as a natural antimicrobial agent. Furthermore, the anticancer potential was evaluated against various cancer cell lines, revealing promising cytotoxic effects, particularly against specific cancer types. Molecular docking studies provided insights into the interaction between bioactive compounds of P. sylvestris extract and key molecular targets implicated in cancer progression, validating its potential as a source of novel anticancer agents. Overall, this comprehensive investigation underscores the pharmacological significance of P. sylvestris extract, emphasizing its therapeutic versatility and potential for drug discovery and development.
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Brody H. Colorectal cancer. Nature. 2015 May 14;521(7551):S1. doi: 10.1038/521S1a. PMID: 25970450.
Patel SG, Karlitz JJ, Yen T, Lieu CH, Boland CR. The rising tide of early-onset colorectal cancer: a comprehensive review of epidemiology, clinical features, biology, risk factors, prevention, and early detection. Lancet Gastroenterol Hepatol. 2022 Mar;7(3):262-274. doi: 10.1016/S2468-1253(21)00426-X. Epub 2022 Jan 26. PMID: 35090605.
Tonini V, Zanni M. Why is early detection of colon cancer still not possible in 2023? World J Gastroenterol. 2024 Jan 21;30(3):211-224. doi: 10.3748/wjg.v30.i3.211. PMID: 38314134; PMCID: PMC10835528.
Modest DP, Pant S, Sartore-Bianchi A. Treatment sequencing in metastatic colorectal cancer. Eur J Cancer. 2019 Mar;109:70-83. doi: 10.1016/j.ejca.2018.12.019. Epub 2019 Jan 25. PMID: 30690295.
Benson AB 3rd, Arnoletti JP, Bekaii-Saab T, Chan E, Chen YJ, Choti MA, Cooper HS, Dilawari RA, Engstrom PF, Enzinger PC, Fleshman JW Jr, Fuchs CS, Grem JL, Knol JA, Leong LA, Lin E, May KS, Mulcahy MF, Murphy K, Rohren E, Ryan DP, Saltz L, Sharma S, Shibata D, Skibber JM, Small W Jr, Sofocleous CT, Venook AP, Willett C; National Comprehensive Cancer Network. Colon cancer. J Natl Compr Canc Netw. 2011 Nov;9(11):1238-90. doi: 10.6004/jnccn.2011.0104. PMID: 22056656.
Chaluvadi SR, Young P, Thompson K, Bahri BA, Gajera B, Narayanan S, Krueger R, Bennetzen JL. Phoenix phylogeny, and analysis of genetic variation in a diverse collection of date palm (Phoenix dactylifera) and related species. Plant Divers. 2018 Dec 18;41(5):330-339. doi: 10.1016/j.pld.2018.11.005. PMID: 31934678; PMCID: PMC6951277.
W. M. Barnes. Proc. Natl. Acad. Sci. USA 91:2216, 1994. (2) N. A. Harrison et al. Plant Dis. 86:676, 2002. (3) N. A. Harrison et al. Ann. Appl. Biol. 153:85, 2008. (4) A. Jeyaprakash and M. A. Hoy. Insect Mol. Biol. 9:393, 2000.
Kuo P, Henderson IR, Lambing C. CTAB DNA Extraction and Genotyping-by-Sequencing to Map Meiotic Crossovers in Plants. Methods Mol Biol. 2022;2484:43-53. doi: 10.1007/978-1-0716-2253-7_4. PMID: 35461443.
Suganthi M, Abirami G, Jayanthi M, Kumar KA, Karuppanan K, Palanisamy S. A method for DNA extraction and molecular identification of Aphids. MethodsX. 2023 Feb 27;10:102100. doi: 10.1016/j.mex.2023.102100. PMID: 36915859; PMCID: PMC10006851.
Lee PY, Costumbrado J, Hsu CY, Kim YH. Agarose gel electrophoresis for the separation of DNA fragments. J Vis Exp. 2012 Apr 20;(62):3923. doi: 10.3791/3923. PMID: 22546956; PMCID: PMC4846332.
Sharma DC, Shukla R, Ali J, Sharma S, Bajpai P, Pathak N. Phytochemical evaluation, antioxidant assay, antibacterial activity and determination of cell viability (J774 and THP1 alpha cell lines) of P. sylvestris leaf crude and methanol purified fractions. EXCLI J. 2016 Feb 5;15:85-94. doi: 10.17179/excli2015-689. PMID: 27047320; PMCID: PMC4817419.
Sheikh ZA, Siddiqui ZA, Naveed S, Usmanghani K. Quantitative Determination of Catechin as Chemical Marker in Pediatric Polyherbal Syrup by HPLC/DAD. J Chromatogr Sci. 2016 Sep;54(8):1324-7. doi: 10.1093/chromsci/bmw078. Epub 2016 May 10. PMID: 27165575.
Azmat S, Zahoor A, Ifzal A, Ahmad VU, Mohammed FV. A new megastigmane glycoside, phoenixoside A, from Phoenix dactylifera. Nat Prod Commun. 2012;7:3–4.
Chowdhury, M. S. H. , Halim, M. , Muhammed, N. , Haque, F. , & Koike, M. (2008). Traditional utilization of wild date palm (Phoenix sylvestris) in rural Bangladesh: An approach to sustainable biodiversity management. Journal of Forestry Research, 19(3), 245–251.
Yan R, Cao Y, Yang B. HPLC-DPPH screening method for evaluation of antioxidant compounds extracted from Semen Oroxyli. Molecules. 2014 Apr 10;19(4):4409-17. doi: 10.3390/molecules19044409. Erratum in: Molecules. 2016;21(1). pii: E125. doi: 10.3390/molecules21010125. PMID: 24727414; PMCID: PMC6271040.
Flieger J, Flieger M. The [DPPH●/DPPH-H]-HPLC-DAD Method on Tracking the Antioxidant Activity of Pure Antioxidants and Goutweed (Aegopodium podagraria L.) Hydroalcoholic Extracts. Molecules. 2020 Dec 18;25(24):6005. doi: 10.3390/molecules25246005. PMID: 33353137; PMCID: PMC7766071.
Urquiza-López A, Álvarez-Rivera G, Ballesteros-Vivas D, Cifuentes A, Del Villar-Martínez AA. Metabolite Profiling of Rosemary Cell Lines with Antiproliferative Potential against Human HT-29 Colon Cancer Cells. Plant Foods Hum Nutr. 2021 Sep;76(3):319-325. doi: 10.1007/s11130-021-00892-w. Epub 2021 Jul 15. PMID: 34264453.
Jahani-Sherafat S, Azimirad M, Ghasemian-Safaei H, Ahmadi Amoli H, Moghim S, Sherkat G, Zali MR. The effect of intestinal microbiota metabolites on HT29 cell line using MTT method in patients with colorectal cancer. Gastroenterol Hepatol Bed Bench. 2019;12(Suppl1):S74-S79. PMID: 32099605; PMCID: PMC7011068.
Hu YL, Wang XB, Chen DD, Guo XJ, Yang QJ, Dong LH, Cheng L. Germanicol induces selective growth inhibitory effects in human colon HCT-116 and HT29 cancer cells through induction of apoptosis, cell cycle arrest and inhibition of cell migration. J BUON. 2016 May-Jun;21(3):626-32. PMID: 27569083.
Kaur T, Madgulkar A, Bhalekar M, Asgaonkar K. Molecular Docking in Formulation and Development. Curr Drug Discov Technol. 2019;16(1):30-39. doi: 10.2174/1570163815666180219112421. PMID: 29468973.
Pinzi L, Rastelli G. Molecular Docking: Shifting Paradigms in Drug Discovery. Int J Mol Sci. 2019 Sep 4;20(18):4331. doi: 10.3390/ijms20184331. PMID: 31487867; PMCID: PMC6769923.
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