Optimization and Hepatoprotective Activity of Herbal Formulation of Methanolic Extracts of Ruta Graveolens and Angelica Sinensis

Abstract

The goal of this study was to evaluate the antioxidant and hepatoprotective effects of RAAS, a specially created herbal mixture that contains methanol extracts of Ruta graveolens and Angelica sinensis. A range of in vitro tests were performed as part of the study to assess any potential health advantages of RAAS. The laboratory assays employed to assess the antioxidant capacity of RAAS included the total antioxidant activity assay, the reducing power assay, and the DPPH radical scavenging assay. These evaluations showed that RAAS has strong antioxidant characteristics, demonstrating its capacity to counteract oxidative stress and disarm dangerous free radicals. Additionally, the study examined the hepatoprotective effects of RAAS utilizing a cellular model that included HepG₂ cells and carbon tetrachloride-induced liver injury (CCl₄). According to the findings, RAAS significantly protected HepG₂ cells from the negative effects of CCl₄-induced liver damage. In conclusion, this study highlights the prospective antioxidant and hepatoprotective properties of RAAS, an herbal formula made from methanol extracts of Ruta graveolens and Angelica sinensis. Strong in vitro antioxidant activity and the capacity to prevent CCl₄-induced damage to liver cells show the potential therapeutic utility of RAAS in reducing oxidative stress and liver-related diseases. Deeper understanding of its uses in boosting liver health and general wellbeing may come from additional studies in the clinical setting and other fields of study