Evaluation Of Antioxidant, ROS Scavenging Activity And Metal Chelation Activity Of Herbal Extracts From Calotropis Gigantea.
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Abstract
The imbalance between free radicals and antioxidants in the body causes oxidative stress, which plays a crucial role in the development of various diseases. Naturally occurring antioxidants derived from plants have emerged as a potentially interesting candidate for lowering oxidative stress due to their capacity to scavenge free radicals. As Calotropis gigantea widely acknowledged to have therapeutic properties, an extensive investigation of its antioxidant potential is required. Therefore, objective of this study is to systematically evaluate the antioxidant activity of different leaf extracts of C. gigantea. The phytochemical composition, antioxidant capacity, and free radical scavenging ability of the plant’s methanol, chloroform, acetone, and aqueous leaf extracts were investigated. The presence of phenols, flavonoids, saponins, tannins, and alkaloids was demonstrated by phytochemical analysis. The high levels of total phenol and flavonoid content, along with substantial amount of reducing activity observed in the reducing assay, suggested that the leaves possessed antioxidant potential. A dose-dependent response by all extracts on DPPH assay confirmed the free radical activity of plant. The primary cause of oxidative stress in our bodies is reactive oxygen species. Our study demonstrates that all plant extracts have a neutralizing effect on superoxide, hydrogen peroxide, and hydroxyl radicals. The study was extended to evaluate the metal chelating capability of plant extracts, which was also found to be dose-dependent. Here, we report that C. gigantea has great promise as a multipurpose therapeutic agent due to its significant antioxidant activity and bioactive components.
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