Effect Of Bacillus Subtilispriming On Growth And Pigment Composition Of Tomato Seedlings (Lycopersicum Esculantum Cv. Pusa Ruby) Under Different Levels Of Polyethylene Glycolstress Conditions

Authors

  • Dr. Kamal Kant Patra
  • Keshamma E
  • Sajeeda Niketh

DOI:

https://doi.org/10.53555/jaz.v45iS1.4536

Keywords:

Tomato, Seed priming, Bacillus subtilis, Growth enhancement, Pigment composition

Abstract

Objective: to explore the quantitative changes in photosynthetic pigments of Lycopersicum esculantum cv. Pusa Rubyseedlings to inoculation with Bacillus subtilis(ATCC No.: 11774) under different levels of polyethylene glycol 6000 (PEG 6000) stress using sustainable techniques such as priming with PGPB strain Bacillus subtilis.

Methods: This study was performed at laboratorycondition with Solanum lycopersicum L. cv. Pusa ruby seeds as factorial experimentunder Randomized Complete Design (CRD) with fourreplications. Effect ofdrought stress induced by different per cent level ofPEG 6000 treatments on drought tolerance in Bacillus subtilis primed tomato seedlings was studied. In this experiment, twentyBacillus subtilis primed tomato (Solanum lycopersicum L. cv. Pusa ruby) seeds were placed in each per cent of PEG mediated drought stress treatment. One set without Bacillus subtilis primed tomato seeds were also treated with different level of PEG 6000 (1, 5, 10, 15, 20, 25, and 30%) mediated drought stress to observe the effect of Bacillus subtilis priming.

Results: Radicle protrusion (%), opening of cotyledonary leaves (%) was increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress at 0-25% and 0-15% respectively. Furthermore, the growth response parameters of viz. fresh weight (g) and dry weight (g) tomato were increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-20%). Chl a andChl b content was higher in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-20%). Furthermore, the carotenoid (mg g-1) quantity was increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-5%). Whereas, the quantity of anthocyanin (mg g-1) was increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-15%).

Conclusion:Bacillus subtilis (ATCC No.: 11774) could be successfully used to enhance fruit production and fruit quality of tomato plants grown under controlled conditions.

Downloads

Download data is not yet available.

Author Biographies

Dr. Kamal Kant Patra

Associate Professor, Department Of Botany, Ybn University, Ranchi,Jharkhand- 834 010, India

 

Keshamma E

Associate Professor, Department Of Biochemistry, Maharani Cluster University, Palace Road, Bengaluru, Karnataka, India

Sajeeda Niketh

Associate Professor, Department Of Botany, Ybn University, Ranchi, Jharkhand, India

References

Vinocur B, Altman A. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Current opinion in biotechnology. 2005;16(2):123-32.

Aggarwal PK, Singh AK. Implications of global climatic change on water and food security. Global change: Impacts on water and food security. 2010:49-63.

Beck EH, Fettig S, Knake C, Hartig K, Bhattarai T. Specific and unspecific responses of plants to cold and drought stress. Journal of biosciences. 2007; 32:501-10.

Samarah NH. Effects of drought stress on growth and yield of barley. Agronomy for sustainable development. 2005;25(1):145-9.

Kamara AY, Menkir A, Badu-Apraku B, Ibikunle O. The influence of drought stress on growth, yield and yield components of selected maize genotypes. The journal of agricultural science. 2003;141(1):43-50.

Lafitte HR, Yongsheng G, Yan S, Li ZK. Whole plant responses, key processes, and adaptation to drought stress: the case of rice. Journal of experimental botany. 2007;58(2):169-75.

Rampino P, Pataleo S, Gerardi C, Mita G, Perrotta C. Drought stress response in wheat: physiological and molecular analysis of resistant and sensitive genotypes. Plant, cell & environment. 2006;29(12):2143-52.

Solanke AU, Sharma AK. Signal transduction during cold stress in plants. Physiology and Molecular biology of plants. 2008;14:69-79.

Urano K, Kurihara Y, Seki M, Shinozaki K. ‘Omics’ analyses of regulatory networks in plant abiotic stress responses. Current opinion in plant biology. 2010 Apr 1;13(2):132-8.

Farooq M, Wahid A, Kobayashi NS, Fujita DB, Basra SM. Plant drought stress: effects, mechanisms and management. Sustainable agriculture. 2009:153-88.

Ahemad M, Khan MS. Effect of insecticide-tolerant and plant growth-promoting Mesorhizobium on the performance of chickpea grown in insecticide stressed alluvial soils. Journal of Crop Science and Biotechnology. 2009;12:217-26.

Chandler D, Davidson G, Grant WP, Greaves J, Tatchell GM. Microbial biopesticides for integrated crop management: an assessment of environmental and regulatory sustainability. Trends in Food Science & Technology. 2008;19(5):275-83.

Warren CR, Adams MA. Evergreen trees do not maximize instantaneous photosynthesis. Trends in plant science. 2004;9(6):270-4.

Wahid A, Gelani S, Ashraf M, Foolad MR. Heat tolerance in plants: an overview. Environmental and experimental botany. 2007;61(3):199-223.

Conrath U, Beckers GJ, Flors V, García-Agustín P, Jakab G, Mauch F, Newman MA, Pieterse CM, Poinssot B, Pozo MJ, Pugin A. Priming: getting ready for battle. Molecular plant-microbe interactions. 2006 Oct;19(10):1062-71.

Lucy M, Reed E, Glick BR. Applications of freeliving plant growth-promoting rhizobacteria. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. 2004;86:1-25.

Dimkpa C, Weinand T, Asch F. Plant–rhizobacteria interactions alleviate abiotic stress conditions. Plant, cell & environment. 2009;32(12):1682-94.

Ashraf MP, Harris PJ. Potential biochemical indicators of salinity tolerance in plants. Plant science. 2004;166(1):3-16.

Creus CM, Sueldo RJ, Barassi CA. Water relations and yield in Azospirillum-inoculated wheat exposed to drought in the field. Canadian Journal of Botany. 2004;82(2):273-81.

Moran R, Porath D. Chlorophyll determination in intact tissues using N, N-dimethylformamide. Plant physiology. 1980;65(3):478-9.

Mancinelli AL, Rabino I. Photocontrol of anthocyanin synthesis: IV. Dose dependence and reciprocity relationships in anthocyanin synthesis. Plant physiology. 1975;56(3):351-5.

Zahir ZA, Arshad M, Frankenberger WT. Plant growth promoting rhizobacteria: Applications and perspectives in agriculture. Advances in Agronomy. 2004; 1(81):98-169.

Ruzzi M, Aroca R. Plant growth-promoting rhizobacteria act as biostimulants in horticulture. Scientia Horticulturae. 2015; 196:124-34.

Kloepper JW, Leong J, Teintze M, Schroth MN. Enhanced plant growth by siderophores produced by plant growthpromoting rhizobacteria. Nature. 1980; 286(5776):885.

Pishchik VN, Chernyaeva II, Kozhemaykov AP, Vorobyov NI, Lazarev AM, Kozlov LP. Effect of inoculation with nitrogen-fixing Klebsiella on potato yield. In Nitrogen Fixation with Non-Legumes. Springer, Dordrecht. 1998;223-235.

Kloepper JW, Ryu CM, Zhang S. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology. 2004;94(11):1259-66.

Ahemad M, Kibret M. Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University-Science. 2014;26(1):1-20.

Gond SK, Bergen MS, Torres MS, White Jr JF. Endophytic Bacillus spp. Produce antifungal lipopeptides and induce host defence gene expression in maize. Microbiological Research. 2015; 172:79-87.

Vessey, J.K. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil. 2003; 255: 571–586.

Zehnder GW, Murphy JF, Sikora EJ, Kloepper JW. Application of rhizobacteria for induced resistance. European journal of plant pathology. 2001; 107:39-50.

Chandrasekaran M, Chun SC. Induction of defence-related enzymes in tomato (Solanum lycopersicum) plants treated with Bacillus subtilis CBR05 against Xanthomonas campestris pv. vesicatoria. Biocontrol Science and Technology. 2016a;26(10):1366-78.

Chandrasekaran M, Chun SC. Expression of PR-protein genes and induction of defense-related enzymes by Bacillus subtilis CBR05 in tomato (Solanum lycopersicum) plants challenged with Erwinia carotovora subsp. carotovora. Bioscience, biotechnology, and biochemistry. 2016b;80(11):2277-83.

Glick BR, Penrose DM, Li J. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. Journal of theoretical biology. 1998;190(1):63-8.

Moco S, Capanoglu E, Tikunov Y, Bino RJ, Boyacioglu D, Hall RD, Vervoort J, De Vos RC. Tissue specialization at the metabolite level is perceived during the development of tomato fruit. Journal of Experimental Botany. 2007;58(15-16):4131-46.

Martí R, Roselló S, Cebolla-Cornejo J. Tomato as a source of carotenoids and polyphenols targeted to cancer prevention. Cancers. 2016;8(6):58.

Ronen G, Cohen M, Zamir D, Hirschberg J. Regulation of carotenoid biosynthesis during tomato fruit development: expression of the gene for lycopene epsilon‐cyclase is down‐regulated during ripening and is elevated in the mutant Delta. The Plant Journal. 1999;17(4):341-51.

Su L, Diretto G, Purgatto E, Danoun S, Zouine M, Li Z, Roustan JP, Bouzayen M, Giuliano G, Chervin C. Carotenoid accumulation during tomato fruit ripening is modulated by the auxin-ethylene balance. BMC plant biology. 2015;15(1):1-2.

Zhang Y, Liu Y, Lv Q. DFT study on the quenching mechanism of singlet oxygen by lycopene. RSC advances. 2016;6(100):98498-505.

Saini RK, Nile SH, Park SW. Carotenoids from fruits and vegetables: Chemistry, analysis, occurrence, bioavailability and biological activities. Food Research International. 2015;76:735-50.

Lagunas J, Zavaleta E, Osada S, Aranda S, Luna I, Vaquera H. Bacillus firmus como agente de control biológico de Phytophthora capsici Leo. en jitomate (Lycopersicon esculentum Mill.). Revista Mexicana de Fitopatología. 2001;19(1):57-65.

Luna Martínez L, Martínez Peniche RA, Hernández Iturriaga M, Arvizu Medrano SM, Pacheco Aguilar JR. Caracterización de rizobacterias aisladas de tomate y su efecto en el crecimiento de tomate y pimiento. Revista fitotecnia mexicana. 2013;36(1):63-9.

Kachigan SK. Multivariate statistical analysis: A conceptual introduction. Radius Press; 1991.

García JA, Probanza A, Ramos B, Palomino M, Manero FJ. Effect of inoculation of Bacillus licheniformis on tomato and pepper. Agronomie. 2004;24(4):169-76.

Kumari I, Kaurav H, Chaudhary G. Eclipta alba (bhringraj): a promising hepatoprotective and hair growth stimulating herb. Asian Journal of Pharmaceutical and Clinical Research. 2021;14(7):16-23.

Choi SH, Kim DS, Kozukue N, Kim HJ, Nishitani Y, Mizuno M, Levin CE, Friedman M. Protein, free amino acid, phenolic, β-carotene, and lycopene content, and antioxidative and cancer cell inhibitory effects of 12 greenhouse-grown commercial cherry tomato varieties. Journal of Food Composition and Analysis. 2014;34(2):115-27.

Saini RK, Zamany AJ, Keum YS. Ripening improves the content of carotenoid, α-tocopherol, and polyunsaturated fatty acids in tomato (Solanum lycopersicum L.) fruits. 3 Biotech. 2017;7:1-7.

Downloads

Published

2024-01-20

Issue

Section

Articles

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.