Effect Of Bacillus Subtilispriming On Growth And Pigment Composition Of Tomato Seedlings (Lycopersicum Esculantum Cv. Pusa Ruby) Under Different Levels Of Polyethylene Glycolstress Conditions
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Abstract
Objective: to explore the quantitative changes in photosynthetic pigments of Lycopersicum esculantum cv. Pusa Rubyseedlings to inoculation with Bacillus subtilis(ATCC No.: 11774) under different levels of polyethylene glycol 6000 (PEG 6000) stress using sustainable techniques such as priming with PGPB strain Bacillus subtilis.
Methods: This study was performed at laboratorycondition with Solanum lycopersicum L. cv. Pusa ruby seeds as factorial experimentunder Randomized Complete Design (CRD) with fourreplications. Effect ofdrought stress induced by different per cent level ofPEG 6000 treatments on drought tolerance in Bacillus subtilis primed tomato seedlings was studied. In this experiment, twentyBacillus subtilis primed tomato (Solanum lycopersicum L. cv. Pusa ruby) seeds were placed in each per cent of PEG mediated drought stress treatment. One set without Bacillus subtilis primed tomato seeds were also treated with different level of PEG 6000 (1, 5, 10, 15, 20, 25, and 30%) mediated drought stress to observe the effect of Bacillus subtilis priming.
Results: Radicle protrusion (%), opening of cotyledonary leaves (%) was increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress at 0-25% and 0-15% respectively. Furthermore, the growth response parameters of viz. fresh weight (g) and dry weight (g) tomato were increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-20%). Chl a andChl b content was higher in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-20%). Furthermore, the carotenoid (mg g-1) quantity was increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-5%). Whereas, the quantity of anthocyanin (mg g-1) was increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-15%).
Conclusion:Bacillus subtilis (ATCC No.: 11774) could be successfully used to enhance fruit production and fruit quality of tomato plants grown under controlled conditions.
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