A Study On Evaluation Of Antidiabetic Properties Of Andrographis Paniculata
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Abstract
Diabetes mellitus is a multifactorial, unavoidable metabolic disease that impairs insulin function or secretion, or both, and causes hyperglycemia. Acarbose and voglibose are still used today as an inhibitor of the enzymes that break down carbohydrates, either by themselves or in conjunction with insulin. However, there have been reports of negative side effects from these compounds, including diarrhea, flatulence, abdominal fullness, and liver disorders.In the present study we aimed to screen for antidiabetic activities of methanol extract of Andrographis paniculatathrough in-vitro evaluation of inhibition of carbohydrate hydrolyzing enzymes such as, alpha-amylase and alpha- glucosidase. Results depicted that methanolic leaf extract of A. paniculata at a concentration range of 20mg/ml, 40mg/ml, 60mg/ml, 80mg/ml and 100mg/ml, shown inhibition effect of 41.57%, 53.48%, 62.28%, 73.87%, and 88.34% respectively with an IC50 value of 56.24 mg/ml in comparison with the standard antidiabetic drug acarbose with an IC50 value of 25.38 mg/ml. Similarly, in an alpha-glucosidase inhibition assay with methanolic leaf extract of A. paniculataat a concentration range of 20mg/ml, 40mg/ml, 60mg/ml, 80mg/ml and 100mg/ml, shown inhibition effect of 18.61%, 29.69%, 34.41%, 49.40%, and 61.82% respectively with an IC50 value of 32.38 mg/ml in comparison with the standard antidiabetic drug acarbose with an IC50 value of 21.89 mg/ml. Furthermore, the phytochemical analysis revealed that total phenolic quantity was found to be highest (54.44 mgGAE/gextract) in methanolic leaf extract of A. paniculata when compared with total flavonoid quantities (0.41 mg QE/g extract). In conclusion, A. paniculata possess anti-diabetic properties in methanol extracts. Hence, A. paniculatacould be explored in development of natural antidiabetic formulations.
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