In-Silico Analysis And Antidiabetic Effect Of Α-Glucosidase Inhibitory Of Active Compounds Of Pterocarpus Marsupium
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Abstract
The identification of anti-diabetic natural compounds is a thriving field of opportunity in the drug discovery sector. P. marsupium has traditionally been used to treat diabetes and contains important phytochemicals. We investigated the TPC, TFC, antioxidant and antidiabetic activities of methanolic extract of P.marsupium. Total amount of phenols, flavonoids in methanolic extract of leave, stem, bark of P.marsupium were found to be 6.25, 12.613, 10.863 µg/ml respectively. The flavonoid content was found in P.marsupium leave (5.043), stem (19.246) and bark (13.782) µg/ml. In DPPH scavenging activity of plants parts found of P.marsupium, IC50 values were found to be leaves (96.146 µg/ml.), stem (21.705.µg/ml) and bark (27.177 µg/ml). Alpha-glucosidase of P.marsupium plants IC50 values were found to be leave (107.77µg/ml), stem (23.64µg/ml) and bark (60.123µg/ml ). In-silico studies were investigated to determine the binding affinity of phytoconstituents of P. marsupium in additional with the crystal structure of alpha-glucosidase (PDB ID: 5ZCD). Thirty one active constituents derived from P.marsupium, and standard (maltotriose) docked with target proteins. Result of molecular docking showed that the pterosupin, (-11.0 kcal/mol) had best docked against alpha-glucosidase as related to native ligand maltoriose (-10.04kcal/mol). This study provides valuable insights into the therapeutic potential of P.marsupium plant compounds for managing diabetes and warrants further research and clinical investigation for practical applications in diabetes treatment and prevention.
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