Anticancer Property Of L-Glutaminase Producing Actinomycete Streptomyces Albogriseolus Isolated From Estuary Of Uttara Kannada District Against Hela And HepG2 Cell Lines.
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Abstract
L-glutaminase (L-glutamine aminohydrolase EC 3.5.1.2) is an extracellular hydrolytic enzyme having anticarcinogenic potential and is widely used in enzyme therapy especially for acute lymphocytic leukemia. L-glutaminase deaminates L-glutamine to glutamic acid and ammonia. In most tumors glutamine is the primary mitochondrial substrate and is present in circulating blood. It is a source of essential amino acid necessary for development of leukemic cells. The lack or depletion of L-glutamine leads to death of tumour cells. The marine environment is a potential source of bioactive secondary metabolites that provides pharmaceutically important compounds. The present study was focussed on to study the anticancer property of L-glutaminase producing actinomycetes isolated from estuaries of Uttara Kannada district of Karnataka. The isolates were screened for L-glutaminase production by qualitative and quantitative assay and the potent isolate was further subjected for MTT assay to determine its anticancer potential. The study showed 60% of isolates were positive for L-glutaminase production in Rapid plate assay and 85% of isolates were positive in semiquantitative assay. In quantitative assay the isolate Streptomyces albogriseolus exhibited high enzyme activity of 24.32+0.02 IU/ml. In MTT assay the isolate Streptomyces albogriseolus showed an IC50 value 102.0μg/ml in cervical cancer HeLa cells and IC50 value of 101.2μg/ml in HePG2 cells respectively, and could be used as good source of L-glutaminase.
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References
Valli, S., Sugasini, S. S., Aysha, O.S., Nirmala, P., Kumar, V. P., and Reena, A. 2012. Antimicrobial potential of Actinomycetes species isolated from marine environment. Asian Pacific Journal of Tropical Biomedicine. 2(6): 469-473.
Haggag, W.W., El Kreem, A.F., and Hoballa, E. 2014. Marine Streptomycetes: Characteristics and Their Antifungal Activities. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 5(5): 651-656.
Teja, D.D., Devi, V.S., Harsha, N., Vishala, S.S., and Lakshmi, P.K.S. 2014. Production of L-glutaminase from marine ecosystems and optimal conditions for maximal production of actinomycetes. International Journal of Advanced Research. 2(1): 485-491.
Rajan, P.C., Mahalakshmipriya, A., Shanmugaraju, V., and Arungandhi, K. 2015. Production of L-glutaminase by marine Streptomyces sp., isolated from West Coast, Kerala, India, World Journal of Applied Biology and Biotechnology Research. 1(1): 38-54.
Lam, K. 2006. Discovery of novel metabolites from marine actinomycetes. Curr opin microbial. 9: 245-251.
Abd – Elnaby, H., Abo – Elala, G., Abdel-Raouf, U., Abd-Elwahab, A., and Hamed, M. 2016. Antibacterial and anticancer activity of marine Streptomyces parvus: optimization and application. Biotechnology and Biotechnological equipment. 30(1): 180-191.
Gulve, R.M., and Deshmukh, A. 2011. Enzymatic activity of actinomycetes isolated from marine sediments. Recent Research in Science and Technology. 3(5): 80-83.
Okazaki, T. and Okami, Y. 1972. Studies on marine microorganisms II Actinomycetes in Sagami Bay and their antibiotic substances. Journal of Antibiotics.25: 461-466.
Jensen, P.R., Dwight, R., and Fenical, W. 1991. Distribution of actinomycetes in near-shore tropical marine sediments. Applied Environmental Microbiology. 29: 1102-1108.
Goodfellow, M., and O’Donnell, A.G. 1989. Search and Discovery of industrially significant actinomycetes In: Baumberg, S., Hunter, I.S., and Rhodes, P.M. Microbial Products: New Approaches. Society for General Microbiology. Cambridge University Press. Cambridge. pp: 343-383.
Lakshmanaperumalsamy, P. 1978. Studies on actinomycetes with special reference to antagonistic Streptomyces from sediments of Porto Novo coastal zone. Ph.D. Thesis, University of Annamalai, India.
Chandrasekaran, M., Keerthi, T.R., and Maity, P. 1998. Antineoplastic activity of L-glutaminase produced by marine Pseudomonas sp., Paper presented in 39th Annual Conference of Association of Microbiologists of India, Mangalore. 5-7.
Athira, R.N., Elizabeth, T., Narendra, T., Ahmed, S.T., Gupta, S.K., Chaudary, M., Siddalingeshwara, K.G., and Pramod, T. 2014. Investigation on the production of L-glutaminase from Pseudomonas stutzeri strain under solid state fermentation using various agro residues. Journal of Drug Delivery and Therapeutics. 4(2):81-85.
Nandakumar, R., Yoshimune, K., Wakayama, M., and Moriguchi, M. 2003. Microbial glutaminase: biochemistry, molecular approaches and applications in the food industry. Journal of Molecular Catalysis B: Enzymatic. 23(2-6): 87-100.
Sarkar, A., Abhyankar, I., Saha, P., Kumar, S.S.R., and Rao, K.V.B. 2014. Antioxidant, haemolytic activity of L-glutaminase producing marine actinobacteria isolated from salt pan soil of coastal Andra Pradesh. Research Journal of Pharmacy and Technology. 7(5): 544-549.
Nathiya, K., Nath, S.S., Angayarkanni, J., and Palaniswamy, M. 2012. In vitro cytotoxicity of L-glutaminase against MCF-7 cell lines. Asian Journal of Pharmaceutical and Clinical Research. 5(2): 171-173.
Sarada, K.V. 2013. Production and applications of L-glutaminase using fermentation technology. Asian Pacific Journal Research. 1: 1-4.
Sabu, A., Sukumaran, R.K., Muthusamy, C. 2002. L-glutaminase as a therapeutic enzyme of microbial origin. Microbial enzymes and Biotransformation. 17: 75-90.
Karim, G.F., and Thalij, K.M. 2016. Determination of L-glutaminase activity by some bacterial species. International Journal of Current Microbiology and Applied Sciences. 5(4): 218-225.
Mesta, S.C., and Onkarappa, R. 2017. Characterization and antimicrobial potential of marine actinomycetes from estuaries of Uttara Kannada District. Karnataka. Journal of Drug Delivery and Therapeutics. 7(6): 27-37.
Gulati, R., Saxena, R.K., and Gupta, R. 1997.A rapid screening for L-asparaginase producing micro-organisms. Letters Appl. Microbiol.24: 23-26.
Balagurunathan, R., Radhakrishnan, M., and Somasundaram, S.T. 2010. L-glutaminase producing actinomycetes from Marine sediments- Selective Isolation, Semi Quantitative Assay and Characterization of Potential Strain. Australian Journal of Basic and Applied Sciences. 4(5):698-705.
Imada, A., Igarasi, S., Nakahama, K., and Isono, M. 1973. Asparaginase and Glutaminase activities of microorganisms. Journal of General Microbiology. 76:85-99.
Yoo, J.C., Han, J.M., Nam, S.K., Ko, O.H., Choi, C.H., Kee, K.H., Sohng, J.K., Jo, J.S., and Seong, C.N. 2002. Characterization and cytotoxic activities of Nonadecanoic acid produced by Streptomyces scabiei sub sp. chosunensis M0137 (KCTC 9927). The Journal of Microbiology. 40 (4): 331-334.
Sudha, S., and Selvam, M.M. 2013. In vitro cytotoxic activity of bioactive metabolite and crude extract from a new actinomycete Streptomyces avidinii strain SU4. International Journal of Pharmacy and Pharmaceutical Sciences. 5(3): 612-616.
Singh, S., Pradhan, N., Kumari, R., and Rao, K.V.B. 2013. Evaluation of antioxidant and cytotoxic activity of bioactive compounds extracted from Nocardiopsis VITSRTB. International Journal of Applied Engineering Research. 8(19): 2203-2208.
Dutt, S.P.L.N.S.N., Siddalingeshwara, K.G., Karthic, J., Pramod, T., and Vishwanatha, T. 2014. Antitumour property L-glutaminase on from Aspergillus oryzae through submerged fermentation. International Journal of Current Microbiology and Applied Sciences. 3(3): 819-823.
Abd-Alla, M.H, El-Sayed, S.A, Rasmey, A.H.M. 2013. Biosynthesis of L-Glutaminase by Streptomyces variabilis ASU319 Isolated from Rhizosphere of Triticum vulgaris. Universal Journal of Microbiology Research. 1(3): 27-35.
Sajitha, N., Vasuki, S., Suja, M., Kokilam, G., and Gopinath, M. 2013. Screening of L-glutaminase from seaweed endophytic fungi. International Research Journal of Pharmaceutical and Applies Sciences (IRJPAS). 3(5): 206-209.
Sathish, T., and Prakasham, R.S. 2012. Influence of Fermentation process parameters on L-Glutaminase production by Bacillus subtilis RSP-GLU. International Journal of Pharmaceutical and Chemical Sciences. 1(3): 974-980.
Suneetha, S., Lakshminarasu, M., Prabhakar, T., and Rao, P.V. 2013. Enhanced L-glutaminase production by newly isolated mutation induced marine Bacillus. International Journal of Inventions in Pharmaceutical Sciences. 1(3): 236-240.
Elshafei, A.M., Hassan, M.M., Ali, N.H., Abouzeid, M.A.E., Mahmoud, D.A., and Elghonemy, D.H. 2014. Purification, Kinetic properties and Anti tumour activity of L-Glutaminase from Penicillium brevicompactum NRC 829. British Microbiology Research Journal. 4(1): 97-115.
Yulianti, T., Chasanah, E., and Tambunan, U.S.F. 2012. Screening and Characterization of L-glutaminase produced by bacteria isolated from Sangihe Talaud sea. Squalen. 7(3): 115-122.
Krishnakumar, S., Rajan, R.A., and Ravikumar, S. 2011. Extracellular production of L-glutaminase by marine alkalophilic Streptomyces sp – SBU1 isolated from Cape Comorin coast. Indian Journal of Geo – Marine Sciences. 40 (5): 717-721.
Desai, S.S., Chopra, S.J., and Hungund, B.S. 2016. Production, purification and characterization of L-glutaminase from Streptomyces sp. isolated from soil. Journal of Applied Pharmaceutical Science. 6(7):100-105.
Wade, H.E. 1980. Synthesis and functions of microbial asparaginases and glutaminases. In: Payne J.W. (ed), John Wilay and Sons, New York. pp 563-575.
Sabu, A. 2003. Sources, properties and applications of microbial therapeutic enzymes. Indian Journal of Biotechnology. 2:334-341.
Dura, M.A., Flores, M., and Toldra, F. 2002. Purification and characterization of L-glutaminase from Debaryomyces spp. International Journal of Food Microbiology. 76: 117-126.
Sivakumar, K., Sahu, M.K., Manivel, P.R., and Kannan, L. 2006. Optimum conditions for L-glutaminase production by actinomycete strain isolated from estuarine fish, Chanos chanos (Forskal, 1775). Indian Journal of Experimental Biology. 44: 256-258.
Abdallah, N.A., Amer, S.K., and Habeeb, M.K. 2013. Production, Purification and Characterization of L-glutaminase enzyme from Streptomyces avermitilis. African Journal of Microbiology Research. 7(14):1184-1190.
Dhevagi, P., and Poorani, E. 2016. Isolation of L-glutaminase producing actinomycetes from Thiruvallum Brackish water. International Journal of Science and Nature. 7(4): 821-827.
Holcenberg, J.S. 1982. Therapy: Problems and Solutions. Ann Rev Biochem. 51: 795-812.
Kumar, S.R., and Chandrasekaran, M. 2003. Continuous production of L-glutaminase by an immobilized marine Pseudomonas sp. BTMS-51 in a packed bed reactor. Proc. Biochem.38: 1431-1436.
Vijayabharathi, R., Bruheim, P., and Andreassen, T. 2011. Assesment of resistomycin as an anticancer compound isolated and characterized from Streptomyces aurantiacus AAA5. Journal of Microbiology. 49(6): 920-926.
Pham, H.T., Nguyen, N.P., Phi, T.Q., Dang, P.T., and Le, H.G. 2014. The antibacterial and anticancer activity of marine actinomycete strain HP411 isolated in the Northern Coast of Vietnam. International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 8(12): 842-846.
Reda, F.M. 2015. Kinetic properties of Streptomyces canarius L-glutaminase and its anticancer efficiency. Brazilian Journal of Microbiology. 46(4): 957-968.
Naine, S.J., Devi, C.S., Mohanasrinivasan, V., and Vaishnavi, B. 2015. Antimicrobial, Antioxidant and Cytotoxic activity of Marine Streptomyces parvulus VITJS11 crude extract. Brazilian Archives of Biology and Technology. 58(2): 198-207.