In Vitro Antioxidant, Antiinflammatory, Antichelating And Anticancer Activity Of Azima Tetracantha Against Hepg2 Cell Line
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Abstract
Medicinal herbs have long been used to treat a wide range of ailments. In the present study, the antioxidant potency of ethanolic extract was investigated, by determining the secondary metabolites such as flavonoids, tannin and phenolic compounds. The free radical scavenging activity of Ethanolic extract of Azima tetracantha (EEAT) was assessed by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide radical (NO), Hydrogen peroxide scavenging assay. Anti-inflammatory property also studied by invitro method such as Inhibition of albumin denaturation and HRBC membrane stabilization. Reducing power also assessed by using Iron chelating property. The Cytotoxicity was investigated against HepG2 cell lines using in vitro techniques such as MTT assay, morphological exams under a light microscope and a fluorescence microscope, DNA fragmentation, and LDH measurement. The results show that the EEAT contains significant antioxidant values. Similar observations were seen in scavenging the free radicals by the EEAT. It exhibits anti-inflammatory and antichelating activity also. Azima tetracantha ethanolic extract had significant cytotoxic activity against HepG2 cells, as measured by cellular death, in a dose-dependent manner. The findings of this study add to our understanding of Azima tetracantha's anticancer activity against HepG2 cells and support its application in the treatment of liver cancer. Thus, the therapeutic property of the plant A. tetracantha can be attributed to its phytoconstituents and its bioactive compounds.
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