Effect of Fermentation Parameters for the Mass Cultivation of Trichoderma viride via Submerged and Solid-State Fermentation Studies On Cellulase Production

Abstract

The present investigation was carried out to evaluate different fermentation parameters and to optimize the fermentation parameters to cultivate Trichoderma viride (T. viride) via submerged and solid-state fermentations. The different parameters such as, effect of pH, temperature, inoculum size, media volume, incubation period and different nitrogen sources.  The highest growth yield noticed was 14.221g L^-1 at 15 g L^-1, 0.15 g L^-1, 40 ^oC, 2.0, 7.0 and 200 carbon, nitrogen, temperature, volume of the medium, pH, RPM respectively. Following, 12.187 g L^-1 yield of T. viride was achieved with 15 g L^-1, 0.15 g L^-1, 40 ^oC, 2.0, 7.0, 200 carbon, nitrogen, temperature, volume of the medium, pH, RPM respectively. the effect of different incubation period on the cellulase activity was ranged from 0.76U/mL/min to 3.02 U/mL/min. The effect of different incubation period on the cellulase activity was ranged from 0.63 U/mL/min to 2.88 U/mL/min. The effect of different pH on the cellulase activity was ranged from 0.436 U/mL/min to 2.481 U/mL/min. the highest cellulase activity was noticed at pH 5.5 with 2.481 Nitrogen sources, five (5) namely, NH₄Cl, NH₄, CH₃COO, (NH₄)₂SO₄, NH₄H₂PO₄, NH₄NO₃ are identified as significant sources for cultivation of T. viride and subsequent production and activity of cellulase.  In accordance to results obtained, the inoculum size of 4mL found significant with 2.321 U/mL/min Out of Seven (7), three (3) media volumes such as 30mL, 40mL, and 50mL produced highest cellulase production with 2.964 U/mL/min, 2.331 U/mL/min.