Bacteriostatic effect of Coumarin 2-(3,4- Dihydroxyphenyl)-3,5,7-Trihydroxy-4H-Chromen-4-One Isolated from the root extract of Strychnos nux vomica.
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Abstract
Introduction and Aim: Strychnos nux-vomica (S. nux-vomica) being Loganiaceae is a well-known herb in India as well in Srilanka, Northern and Southeast Asia America. The present investigation has been carried out to evaluate the bacteriostatic effects of 2-(3,4- dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one isolated from root ethyl acetate extract of S. nux-vomica.
Materials and Methods: Structure elucidation was carried out using 1HNMR.The bacteriostatic effect of isolated compound at 75 and 100 µg/ml was evaluated using agar well diffusion method against MRSA (Methicillin Resistant Staphylococcus aureus, B. subtilis, B. cereus, P.aeruginosa, E.coli, S. typhi.
Result: Based on the result, we noted that, MRSA was most susceptible bacterial strain with 5.4mm and 11.3mm zone of inhibitions recorded at 75 and 100µg/ml respectively. Result is compared with known standard gentamycin sulphate.
Conclusion: In accordance to the result of the investigation, here we conclude that, the isolated compound is coumarin derivative compound and it exhibit significant activity against MRSA.
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