Testicular Dysfunction Via Uvb-Induced Hyperthyroidism And Prophylactic Glycyrrhizin Dose In Swiss Albino Mice
DOI:
https://doi.org/10.53555/jaz.v45i6.4833Keywords:
Hyperthyroidism, Radiations, Testicular dysfunction, GlycyrrhizinAbstract
This study explores the protective potential of glycyrrhizin, a compound from licorice root, against UVB-induced testicular dysfunction. UVB exposure disrupts hormonal balance, potentially leading to impaired male fertility. Glycyrrhizin's established antioxidant and anti-inflammatory properties suggest its efficacy in mitigating these detrimental effects.
A rodent model will be employed, with controlled UVB irradiation mimicking hyperthyroidism-induced testicular damage. Testicular function will be assessed via sperm quality and motility. Additionally, oxidative stress markers (malondialdehyde) and antioxidant enzyme activity (SOD, CAT, GSH) will be evaluated. Histological examination will reveal potential alterations in spermatogenesis and seminiferous tubule structure.
A crucial aspect involves administering glycyrrhizin to a designated UVB-exposed group. Comparing parameters between control, UVB-exposed, and glycyrrhizin-treated groups will elucidate its potential to reduce oxidative stress and preserve testicular health.
This research aims to shed light on glycyrrhizin's protective role against UVB-induced testicular toxicity. By evaluating its efficacy in mitigating oxidative damage and preserving testicular function, this study can contribute valuable insights for developing therapeutic strategies to safeguard male reproductive health following UVB exposure. The findings may hold particular relevance for individuals with high occupational UVB exposure or undergoing phototherapy treatments utilizing UVB radiation.
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1. Abo-Elnour, R.K. and El-Deeb, D.F., 2012. A histological study on the effect of experimentally induced hyperthyroidism on adult albino rat testis. Egyptian Journal of Histology, 35(4), pp.862-871.
2. Akindele, A.J., Adeneye, A.A., Salau, O.S., Sofidiya, M.O. and Benebo, A.S., 2014. Dose and time-dependent sub-chronic toxicity study of hydroethanolic leaf extract of Flabellaria paniculata Cav.(Malpighiaceae) in rodents. Frontiers in pharmacology, 5, p.78.
3. Alahmar, A., Dutta, S. and Sengupta, P., 2019. Thyroid hormones in male reproduction and infertility. Asian Pacific Journal of Reproduction, 8(5), pp.203-210.
4. Asayama, K. and Kato, K., 1990. Oxidative muscular injury and its relevance to hyperthyroidism. Free Radical Biology and Medicine, 8(3), pp.293-303.
5. Asker, M.E., Hassan, W.A. and El‐Kashlan, A.M., 2015. Experimentally induced hyperthyroidism influences oxidant and antioxidant status and impairs male gonadal functions in adult rats. Andrologia, 47(6), pp.644-654.
6. Azenabor, A., Ekun, A.O. and Akinloye, O., 2015. Impact of inflammation on male reproductive tract. Journal of reproduction & infertility, 16(3), p.123.
7. Babu, K., Jayaraaj, I.A. and Prabhakar, J., 2011. Effect of Abnormal thyroid hormone changes in lipid peroxidation and Antioxidant imbalance in Hypothyroid and Hyperthyroid patients. Int J Biol Med Res, 2(4), pp.1122-1126.
8. Baghel, K. and Srivastava, R., 2021. Photoperiod dependent expression of estrogen receptor alpha in testes of Japanese quail: Involvement of Withania somnifera in apoptosis amelioration. Biochemical and Biophysical Research Communications, 534, pp.957-965.
9. Baydas, B. and Meral, I., 2005. Effects of melatonin on lipid peroxidation and anti‐oxidant enzyme activity in rats with experimentally induced hyperthyroidism. Clinical and experimental pharmacology and physiology, 32(7), pp.541-544.
10. Beauchamp, C. and Fridovich, I., 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical biochemistry, 44(1), pp.276-287.
11. Bergmeyer HU. Methods of enzymatic analysis. Weinheim, Germany: Verlag Chemie 1983. 3, 3 273-286.
12. Bhattacharyya, A. and Wiles, P.G., 1997. Thyrotoxic crisis presenting as acute abdomen. Journal of the Royal Society of Medicine, 90(12), pp.681-682.
13. Cadet, J., Douki, T. and Ravanat, J.L., 2015. Oxidatively generated damage to cellular DNA by UVB and UVA radiation. Photochemistry and photobiology, 91(1), pp.140-155.
14. Carlberg, I.N.C.E.R. and Mannervik, B.E.N.G.T., 1975. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. Journal of biological chemistry, 250(14), pp.5475-5480.
15. Chakraborty, A., Singh, V., Singh, K. and Rajender, S., 2020. Excess iodine impairs spermatogenesis by inducing oxidative stress and perturbing the blood testis barrier. Reproductive Toxicology, 96, pp.128-140.
16. Chattopadhyay, S., Choudhury, S., Roy, A., Chainy, G.B. and Samanta, L., 2010. T3 fails to restore mitochondrial thiol redox status altered by experimental hypothyroidism in rat testis. General and Comparative Endocrinology, 169(1), pp.39-47.
17. Chattopadhyay, S., Sahoo, D.K., Subudhi, U. and Chainy, G.B.N., 2007. Differential expression profiles of antioxidant enzymes and glutathione redox status in hyperthyroid rats: a temporal analysis. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 146(3), pp.383-391.
18. Choudhury, S., Chainy, G.B.N. and Mishro, M.M., 2003. Experimentally induced hypo‐and hyper‐thyroidism influence on the antioxidant defence system in adult rat testis. Andrologia, 35(3), pp.131-140.
19. Dantzer, F., Mark, M., Quenet, D., Scherthan, H., Huber, A., Liebe, B., Monaco, L., Chicheportiche, A., Sassone-Corsi, P., de Murcia, G. and Ménissier-de Murcia, J., 2006. Poly (ADP-ribose) polymerase-2 contributes to the fidelity of male meiosis I and spermiogenesis. Proceedings of the National Academy of Sciences, 103(40), pp.14854-14859.
20. Darbandi, M., Darbandi, S., Agarwal, A., Sengupta, P., Durairajanayagam, D., Henkel, R. and Sadeghi, M.R., 2018. Reactive oxygen species and male reproductive hormones. Reproductive Biology and Endocrinology, 16, pp.1-14.
21. Dasdag, S., Akdag, M.Z., Erdal, M.E., Erdal, N., Ay, O.I., Ay, M.E., Yilmaz, S.G., Tasdelen, B. and Yegin, K., 2015. Effects of 2.4 GHz radiofrequency radiation emitted from Wi-Fi equipment on microRNA expression in brain tissue. International journal of radiation biology, 91(7), pp.555-561.
22. Dowlath, M.J.H., Karuppannan, S.K., Sinha, P., Dowlath, N.S., Arunachalam, K.D., Ravindran, B., Chang, S.W., Nguyen-Tri, P. and Nguyen, D.D., 2021. Effects of radiation and role of plants in radioprotection: A critical review. Science of the Total Environment, 779, p.146431.
23. Dutta, S., Sengupta, P., Slama, P. and Roychoudhury, S., 2021. Oxidative stress, testicular inflammatory pathways, and male reproduction. International journal of molecular sciences, 22(18), p.10043.
24. Elbers, L.P., Moran, C., Gerdes, V.E., van Zaane, B., Meijers, J.C., Endert, E., Lyons, G., Chatterjee, K., Bisschop, P.H. and Fliers, E., 2016. The hypercoagulable state in hyperthyroidism is mediated via the thyroid hormone β receptor pathway. European journal of endocrinology, 174(6), pp.755-762.
25. Faraone-Mennella, M.R., Ferone, A., Marino, L., Cardone, A., Comitato, R., Venditti, P., Di Meo, S. and Farina, B., 2009. Poly (ADP-ribosyl) ation of proteins and germ cell development in hyperthyroid rat testes. Molecular and cellular biochemistry, 323, pp.119-129.
26. Gown, A.M. and Willingham, M.C., 2002. Improved detection of apoptotic cells in archival paraffin sections: immunohistochemistry using antibodies to cleaved caspase 3. Journal of Histochemistry & Cytochemistry, 50(4), pp.449-454.
27. Griess, P., 1879. Bemerkungen zu der Abhandlung der HH. Weselsky und Benedikt „Ueber einige Azoverbindungen” ︁. Berichte der deutschen chemischen Gesellschaft, 12(1), pp.426-428.
28. Hassan, G.S. and Zahran, D.H., 2019. Effects of experimentally induced hyperthyroidism on rat tongue mucosa: Histological and ultrastructural study. Life Science Journal, 16(1), pp.127-134.
29. Ikegami, K., Refetoff, S., Van Cauter, E. and Yoshimura, T., 2019. Interconnection between circadian clocks and thyroid function. Nature Reviews Endocrinology, 15(10), pp.590-600.
30. Jannini, E.A., Crescenzi, A., Rucci, N., Screponi, E., Carosa, E., De Matteis, A., Macchia, E., d’Amati, G. and D’Armiento, M., 2000. Ontogenetic pattern of thyroid hormone receptor expression in the human testis. The Journal of Clinical Endocrinology & Metabolism, 85(9), pp.3453-3457.
31. Jannini, E.A., Ulisse, S. and D'Armiento, M., 1995. Thyroid hormone and male gonadal function. Endocrine reviews, 16(4), pp.443-459.
32. Jayaprakasam, B., Doddaga, S., Wang, R., Holmes, D., Goldfarb, J. and Li, X.M., 2009. Licorice flavonoids inhibit eotaxin-1 secretion by human fetal lung fibroblasts in vitro. Journal of agricultural and food chemistry, 57(3), pp.820-825.
33. Jayaprakasam, B., Doddaga, S., Wang, R., Holmes, D., Goldfarb, J. and Li, X.M., 2009. Licorice flavonoids inhibit eotaxin-1 secretion by human fetal lung fibroblasts in vitro. Journal of agricultural and food chemistry, 57(3), pp.820-825.
34. Kalyanaraman, B., Darley-Usmar, V., Davies, K.J., Dennery, P.A., Forman, H.J., Grisham, M.B., Mann, G.E., Moore, K., Roberts II, L.J. and Ischiropoulos, H., 2012. Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. Free radical biology and medicine, 52(1), pp.1-6.
35. Karimani, A., Heidarpour, M. and Moghaddam Jafari, A., 2019. Protective effects of glycyrrhizin on sub-chronic diazinon-induced biochemical, hematological alterations and oxidative stress indices in male Wistar rats. Drug and chemical toxicology, 42(3), pp.300-308.
36. Ko, E.Y., Sabanegh Jr, E.S. and Agarwal, A., 2014. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertility and sterility, 102(6), pp.1518-1527.
37. Kopeina, G.S., Prokhorova, E.A., Lavrik, I.N. and Zhivotovsky, B., 2018. Alterations in the nucleocytoplasmic transport in apoptosis: Caspases lead the way. Cell Proliferation, 51(5), p.e12467.
38. Krajewska-Kulak, E., & Sengupta, P. 2013. Thyroid function in male infertility. Frontiers in endocrinology, 4, 174.
39. Kumar, A., Shekhar, S., & Dhole, B. 2014. Thyroid and male reproduction. Indian Journal of Endocrinology and Metabolism, 18(1), 23.
40. La Vignera, S., & Vita, R. 2018. Thyroid dysfunction and semen quality. International journal of immunopathology and pharmacology, 32, 2058738418775241.
41. Li, X. L., Zhou, A. G., Zhang, L., & Chen, W. J. 2011. Antioxidant status and immune activity of glycyrrhizin in allergic rhinitis mice. International journal of molecular sciences, 12(2), 905-916.
42. Liu, Q., Si, T., Xu, X., Liang, F., Wang, L., & Pan, S. 2015. Electromagnetic radiation at 900 MHz induces sperm apoptosis through bcl-2, bax and caspase-3 signaling pathways in rats. Reproductive Health, 12(1), 1-9.
43. Malekinejad, H., Mirzakhani, N., Razi, M., Cheraghi, H., Alizadeh, A., & Dardmeh, F. 2011. Protective effects of melatonin and Glycyrrhiza glabra extract on ochratoxin A—induced damages on testes in mature rats. Human & experimental toxicology, 30(2), 110-123.
44. Maran, R. R. M. 2003. Thyroid hormones: their role in testicular steroidogenesis. Archives of andrology, 49(5), 375-388.
45. Mennella, M. R. F., Roma, G., & Farina, B. 2003. Active poly (ADPribose) metabolism in DNAase‐and salt‐resistant rat testis chromatin with high transcriptional activity/competence. Journal of cellular biochemistry, 89(4), 688-697.
46. Mircescu, G. 2008. Oxidative Stress of Chronic Kidney Disease. Acta Endocrinologica, 4(4), 1841-0987.
47. Napoli, C., Paolisso, G., Casamassimi, A., Al-Omran, M., Barbieri, M., Sommese, L., ... & Ignarro, L. J. 2013. Effects of nitric oxide on cell proliferation: novel insights. Journal of the American College of Cardiology, 62(2), 89-95.
48. Narayana, K., D Souza, U. J., & Rao, K. S. 2002. Effect of ribavirin on epididymal sperm count in rat. Indian journal of physiology and pharmacology, 46(1), 97-101.
49. Noh, S., Go, A., Kim, D. B., Park, M., Jeon, H. W., & Kim, B. 2020. Role of antioxidant natural products in management of infertility: a review of their medicinal potential. Antioxidants, 9(10), 957.
50. Orazizadeh, M., Khorsandi, L., Mansouri, E., & Fakhredini, F. 2020. The effect of glycyrrhizin acid on Bax and Bcl2 expression in hepatotoxicity induced by Titanium dioxide nanoparticles in rats. Gastroenterology and Hepatology from Bed to Bench, 13(2), 168.
51. Placer, Z.A., Cushman, L.L. and Johnson, B.C., 1996. Estimation of lipid peroxidation, malindialdehyde in biochemical system. Anal. Biochem, 16, pp.359-367.
52. Rago, R., & Salacone, P. 2008. Hypothalamus-hypophysis-testicle axis. Andrological Sciences, 15(1), 13-17.
53. Rai, G., & Mahobiya, P. 2022. Eventual study of ultraviolet B radiation and their peril on thyroid gland of female Wistar rat. International Journal of Radiation Research, 20(2), 507-510.
54. Rai, S. P., & Vijayalaxmi, K. K. 2001. Tamoxifen citrate induced sperm shape abnormalities in the in vivo mouse. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 492(1-2), 1-6.
55. Ramadan, H. M., Taha, N. A., & Ahmed, H. H. 2021. Melatonin enhances antioxidant defenses but could not ameliorate the reproductive disorders in induced hyperthyroidism model in male rats. Environmental Science and Pollution Research, 28, 4790-4804.
56. Rao, J. N., Liang, J. Y., Chakraborti, P., & Feng, P. 2003. Effect of thyroid hormone on the development and gene expression of hormone receptors in rat testes in vivo. Journal of endocrinological investigation, 26, 435-443.
57. Rijntjes, E., Wientjes, A. T., Swarts, H. J., de Rooij, D. G., & Teerds, K. J. 2008. Dietary‐induced hyperthyroidism marginally affects neonatal testicular development. Journal of andrology, 29(6), 643-653.
58. Sachidhanandam, M., Singh, S.N., Salhan, A.K. and Ray, U.S., 2010. Evaluation of plasma hormone concentrations using Enzyme-Immunoassay/Enzyme-linked Immunosorbent assay in healthy Indian men: Effect of ethnicity. Indian Journal of Clinical Biochemistry, 25, pp.153-157.
59. Sahoo, D. K. 2012. Alterations of testicular selenium-dependent and independent glutathione peroxidase activities during experimentally L-thyroxine induced hyperthyroidism and n-propyl thiouracil induced hypothyroidism in adult rats. Research & Reviews in BioSciences, 6(3).
60. Sahoo, D. K., Roy, A., Bhanja, S., & Chainy, G. B. N. 2005. Experimental hyperthyroidism-induced oxidative stress and impairment of antioxidant defense system in rat testis. Indian journal of Experimental Biology, 43, 1058-1067.
61. Sakr, S. A., & Shalaby, S. Y. 2014. Carbendazim-induced testicular damage and oxidative stress in albino rats: ameliorative effect of licorice aqueous extract. Toxicology and Industrial Health, 30(3), 259-267.
62. Sedlak, J. and Lindsay, R.H., 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical biochemistry, 25, pp.192-205.
63. Shakyawal, S. and Mahobiya, P. 2023. Repeated UVB Radiations Induced Thyroid Alteration and Oxidative Stress in Male Swiss albino Mice. American Journal of Biomedical Science & Research, 28(4).
64. Shakyawal, S., Namdev, N., Ahmad, Z., & Mahobiya, P. 2023. Effects of Ultraviolet B Radiation on the Function of the Testicles, Expression of Caspase-3 and NOS-2, and the Protective Role of Naringin in Mice. Reproductive Sciences, 31(2), 452-468.
65. Sharpe, R. M. 2010. Environmental/lifestyle effects on spermatogenesis. Philosophical Transactions of the Royal Society B: Biological Sciences, 365(1546), 1697-1712.
66. Singh, R., J Hamada, A., & Agarwal, A. 2011. Thyroid hormones in male reproduction and fertility. The Open Reproductive Science Journal, 3(1).
67. Sinha, A.K., 1972. Colorimetric assay of catalase. Analytical biochemistry, 47(2), pp.389-394.
68. Spaliviero, J. A., Jimenez, M., Allan, C. M., & Handelsman, D. J. 2004. Luteinizing hormone receptor-mediated effects on initiation of spermatogenesis in gonadotropin-deficient (hpg) mice are replicated by testosterone. Biology of reproduction, 70(1), 32-38.
69. Stefanini, M., Martino, C.D. and Zamboni, L., 1967. Fixation of ejaculated spermatozoa for electron microscopy. Nature, 216(5111), pp.173-174.
70. Venditti, P., & Meo, S. D. 2006. Thyroid hormone-induced oxidative stress. Cellular and Molecular Life Sciences CMLS, 63, 414-434.
71. Wagner, M. S., Wajner, S. M., & Maia, A. L. 2008. The role of thyroid hormone in testicular development and function. The Journal of endocrinology, 199(3), 351.
72. Wajner, S. M., Wagner, M. S., & Maia, A. L. 2009. Clinical implications of altered thyroid status in male testicular function. Arquivos Brasileiros de Endocrinologia & Metabologia, 53, 976-982.
73. Wang, C., Jin, Y., & Jin, Y. 2016. Promoting effect of licorice extract on spermatogonial proliferation and spermatocytes differentiation of neonatal mice in vitro. In Vitro Cellular & Developmental Biology-Animal, 52, 149-155.
74. Xiao, Y., Xu, J., Mao, C., Jin, M., Wu, Q., Zou, J., ... & Zhang, Y. 2010. 18β-glycyrrhetinic acid ameliorates acute Propionibacterium acnes-induced liver injury through inhibition of macrophage inflammatory protein-1α. Journal of Biological Chemistry, 285(2), 1128-1137.
75. Yakymenko, I., Tsybulin, O., Sidorik, E., Henshel, D., Kyrylenko, O., & Kyrylenko, S. 2016. Oxidative mechanisms of biological activity of low-intensity radiofrequency radiation. Electromagnetic biology and medicine, 35(2), 186-202.
76. Yogianti, F., Kunisada, M., Nakano, E., Ono, R., Sakumi, K., Oka, S., ... & Nishigori, C. 2014. Inhibitory effects of dietary Spirulina platensis on UVB-induced skin inflammatory responses and carcinogenesis. Journal of Investigative Dermatology, 134(10), 2610-2619.
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