A Preliminary Screening And Determination Of Biochemical Properties Of Bacterial Isolates Producing An Anticancerous Enzyme Asparaginase From Some Sewage Water Samples Of Bhopal, M.P. India

Abstract

Microorganisms have had a major impact on the development of medical science since the discovery that they not only cause infections but also produce certain organic compounds that cure infections and help treat a variety of non-infectious diseases Though, microbes are ubiquitous, but their metabolic capabilities are greatly influenced by the habitat they survive with unique conditions of pH, temperature, pressure, oxygen, light, nutrients and salinity, there is a high efficiency for those to yield metabolites to exhibit special biological activities The production of enzymes is a pursuit central to the modern biotechnology industry. Asparaginase is one such important enzyme finds their use in the pharmaceutical, biosensor and food industries and has anticarcinogenic potential for the treatment of acute lymphoblastic leukemia, lymphomas and other cancers. Asparaginase are naturally occurring enzymes expressed and produced by animal tissues, bacteria, plants, and in the serum of certain rodents, but not in mankind. Erwinia carotovora, Pseudomonas stutzeri, Pseudomonas aeruginosa, E. coli etc has been known to produce L-asparaginase, though its commercial production is restricted to use of Erwinia chrysanthemi and E. coli as per the latest information. Thus the present work was intended to screen out certain new bacterial isolates with in vitro asperginase producing potential from sewage water sources. The sewage water samples were collected from 5 different location of Bhopal and were subjected to brain heart infusion media containing amino acid asparagine and phenol red indicator. The bacterial isolates forming pink or red colonies or hallow around indicating asparaginase producing capability were picked and pure cultured. The three asparaginase positive isolates one from M.P. Nagar & other two from AIIMS sewage sample reported to be bacillus and coccus respectively on Gram’s staining which when subjected to biochemical characterization using catalase test, IMVC test and carbohydrate fermentation tests does not matches with the biochemical characteristics of standard E.coli and S. aureus. The outcomes of this preliminary screening encourages to further investigate the sewage water samples to screen out the possibly new & efficient asparaginase producing isolates with complete biochemical & molecular characterization in view of commercial prospects.