Standardization And Analytical Evaluation Of Siddha Herbal Formulation Aavarai Kirutham In The Treatment Of Madhumega Avathai Vii: Diabetic Peripheral Neuropathy

Abstract

Aavaarai kirutham is a Siddha herbal confection which literarily recommended for the treatment of diabetic peripheral neuropathy (Mathumega Avathaigal) in the authentic Siddha textbook " Sigicha rathina deepam". There are no standards for this antidiabetic herbal preparation. Standardization of the individual ingredients helps to prepare a quality test drug which responsible for the desired pharmacological effect. So, the present study has been performed to standardize unique ingredients and the herbal formulation 'Aavaarai kirutham' as a preliminary study by using three groups of test drug samples. The parameters used to standardize the preparation are organoleptic characters, Biochemical analysis, Physicochemical properties,HPTLC fingerprint analysis, Heavy metal analysis by ASS, Test for specific pathogens, Sterility test by pour plate method, Aflotoxin analysis, Pesticide residue analysis,and Thin layer chromatography. The organoleptic characteristics such as color, taste, odor, smell, and consistency of the ingredients and the test drug were evaluated. Physico-chemical parameters were determined as per WHO guidelines and reported as total ash, insoluble acid ash, water soluble extractive, ethanol soluble extractive, total sugar, reducing sugar content, fat content, and loss on drying. Thin layer chromatography developed for different solvent systems and detection of TLC fingerprint profiles were carried out under UV light at the wavelength of 366nm and also viewed after spraying with anisaldehyde sulfuric acid spray reagent. Colors and Rf Values of major spots were noted.