Evaluation Of Anti-Cataract Activity Of The Siddha Formulation Muthu Parpam Using Isolated Goat Lens Model

Abstract

Siddha system recommends a number of herbal and herbomineral ophthalmic formulations as both internal and external therapeutic measures. Muthu parpam (MPM) is one such therapeutic measure that has been indicated in several eye formulations as an ingredient. While Muthuchippi parpam (Calcined shell of pearl oyster)) has been previously evaluated for its antioxidant profile, till date no study has been performed on anticataract activity of Siddha formulation Muthu parpam (Calcined Pearl). In the present study, the Aldose reductase  inhibitory activity of Muthu parpam (MPM) were studied along with their effect on sugar-induced cataractogenic changes in sheep lenses in vitro. Goat eye lenses were divided into 4 groups; Group I served as normal control, Group II as cataract control, Group III (100μg/ml of MPM) and Group IV(200 μg/ml of MPM). Group II, III and IV were incubated in 55 mM glucose in artificial aqueous humor to induce lens opacification. Freshly prepared aldoreductase enzyme was allowed to react with different volume of test drug at 100, 200,300, 400 and 500µg/and the formation of NADP was observed and the results were expressed as percentage inhibition and were calculated as follows. A dose dependent action of the drug was observed in two groups (III & IV) incubated with 100 μg and 200 μg of the test drug with a visibility scoring of 13.8 ± 3.34 and 22.2  ± 6.14 respectively. It was observed from the results of the present investigation that the Siddha formulation MPM shown significant inhibition of aldose reductase enzyme with the maximum inhibition of about 45.41± 1.761 % and the corresponding IC50 is 558.1 ± 29.79 μg /ml.