Investigation of toxogenic saxitoxin cyanobacteria by PCR method in Hormozgan province, Persian Gulf, Iran
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Abstract
Introduction: Toxic cyanobacteria include Anabaena flos-aquae, Microcystis aeruginosa, Oscillatoria, and Planktotherix. Cyanotoxins fall into four categories: neurotoxin, hepatotoxin, cytotoxin, dermototoxin .The more critical toxins in Cyanobacteria are saxitoxin. It affects the nervous system and respiration. The cyanobacteria produce toxins that put water health at risk. This poison is one of the neurotoxins that is transmitted through the nervous system. The best-known poison is the paralysis of crustaceans (PST). Materials and Methods: This Study aims to identify saxitoxin-producing cyanobacteria in Hormozgan in the Persian Gulf rapidly. Twenty water samples were collected from different stations in the Persian Gulf. DNA extracted by modified DNG method kit. The standard DNA toxogenic strains of Anabaena circinalis (AWQC131C) optimized polymerase chain reaction (PCR) tests. They subsequently evaluated them for specificity and sensitivity. Amplicon in R57PTZ plasmid for sequencing and T-positive control A cloning method optimized the PCR test. Results: Nine out of twenty samples were collected at different stations observed. This study shows the stations located in the estuaries (Tiab, Darsarokh, Jalabi) contain saxitoxin-producing cyanobacteria. Conclusion: In the estuary areas, there is a lot of urban and industrial sewage, which increases harmful cyanobacteria, and with the increase of cyanobacteria, fish die, fishing, economic problems, and pollution increase. Contamination of crustaceans and fish has caused human poisoning, so we must prevent sewage from entering the estuary to avoid the rise of cyanobacteria and toxin production.
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