Heavy Metal Resistant Bacteria Isolation from Koodankulam Coast: Bacillus sp. and Pseudomonas sp. as Potential Agents for Bioremediation
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Abstract
The present study investigates the heavy metal resistant bacteria were isolated from offshore sea soil of Koodankulam coast, Gulf of Mannar, South East coast of India., Tamil Nadu, India. Bacterial communities in the soil were screened by morphological, biochemical and plasmid analysis and were identified as Bacillus sp., and Pseudomonas sp. High concentrations of toxic metals negatively affect bacterial growth, and therefore, the minimum inhibitory concentration of isolated bacteria was determined against Pb, Hg and Zn by agar dilution technique. The heavy metal polluted soil contains some microorganisms that exhibit tolerance to the metals which in turn helps in the reduction of heavy metals toxicity accumulated in the soil. Isolation, screening and characterization are involved to determine the level of resistance of the bacteria. Here, out of several isolates the Bacillus sp. and Pseudomonas sp. has showed increased resistance to Pb, Hg and Zn. Consequently, this microbial isolate can be potentially used in bioremediation of heavy metal polluted environment.
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