Effects of Stocking Density on the Growth Performance, Physiological Parameters and Antioxidant Status of Juvenile Common Carp (Cyprinus carpio L.) Reared in the Cage system

Main Article Content

Sana Suran Dunun
Samad Sofy Omar

Abstract

Stocking density is one of the most important factors influencing growth performance, health status and welfare of fish in aquaculture. This research was carried out to investigate the effect of different stocking densities on growth performance, feed utilization, survival rate, haematology, serum biochemistry and serum antioxidants enzyme responses in common carp juveniles (Cyprinus carpio L.) reared in outdoor cage culture. The fish were randomly stocked in four density groups; 7F fish/0.2m3 (Control group), 14F fish/0.2m3, 21F fish/0.2m3 and 28F fish/0.2m3 in 12 cylindrical cages (0.2m3) in concrete pond, with three cages in each density for 84 days. The results of the 14f density group significantly improved the FW, WG, SGR, FCR, FCE, PER, FI, PI, WBC, RBC, Hct, GSH and SOD activities. The 21F and 28F had significant impacts on FW, WG, SGR, FCR, FCE, PER, FI, PI, glucose, cortisol, ALT, AST, ALP, TG, MDA and GSH increased significantly with increasing stocking density. In contrast, the survival rate, TP, TG, HDL, GPx and SOD activities were significantly decreased. In general, the results demonstrated that the best stocking density is 14F fish/0.2m3 which provided a better growth performance and health status of C. carpio cultured in the cage in the concrete pond.

Downloads

Download data is not yet available.

Article Details

How to Cite
Sana Suran Dunun, & Samad Sofy Omar. (2024). Effects of Stocking Density on the Growth Performance, Physiological Parameters and Antioxidant Status of Juvenile Common Carp (Cyprinus carpio L.) Reared in the Cage system. Journal of Advanced Zoology, 45(2), 1044–1053. https://doi.org/10.53555/jaz.v45i2.3870
Section
Articles
Author Biographies

Sana Suran Dunun

Fish Resources and Aquatic Animals Department, College of Agriculture Engineering Sciences, Salahaddin University, Erbil, Kurdistan Region, Iraq.

Samad Sofy Omar

Biology Education Department, Tishk International University, Kurdistan Region, Iraq

References

Maragoudaki, D., Paspais, M. and Kentouri, M. (1999) Influence of Stocking Density of Juvenile Red Porgy (Pagrus pagrus ) under Different Feeding Conditions. Aquaculture Research, 30, 501-508. https://doi.org/10.1046/j.1365-2109.1999.00363.x

El-Saidy, D.M.S. and Gaber, M.M. (2002) Intensive Culture of Nile tilapia , Oreochromis niloticus (L), in Concrete Tanks in Egypt: Effect of Stocking and Feeding Levels on Growth Performance, Production Traits, Feed Conversion and Body Composition. First Scientific Conference of the Egyptian Aquaculture Society , Organized by Faculty of Environmental Agriculture Sciences, Suez Canal University, El-Arish-North Sinai, Egypt.

Abdel-Tawwab, M., Hagras, A.E., Elbaghdady, H.A.M., Monier, M.N., 2014. Dissolved oxygen level and stocking density effects on growth, feed utilization, physiology, and innate immunity of Nile tilapia, Oreochromis niloticus. J. Appl. Aquacult. 26 (4), 340-355.

Abutalb, Ahmed A. Hussein, Mohsen S., Gewida Ahmed G. A., Abdelghny Mohamed F. (2022). Effect of different feeding times on growth performance and blood parameters of African catfish (Clarias gariepinus) under different stocking densities. Al-Azhar Journal of Agricultural Research. DOI: 10.21608/ajar.2023.159524.1089

Abu Zafar, Moshabberul Alam Roni, Masud Rana & Nahid Akter (2023) Growth, digestive enzyme activities, proximate composition and hemato-biochemcial responses of juvenile Nile tilapia (Oreochromis niloticus) reared at various stocking densities in a recirculatory aquaculture system, Journal of Applied Aquaculture, 35:4, 1179-1201, DOI: 10.1080/10454438.2022.2102957

Andrade, T., Afonso, A., Pérez‐Jiménez, A., Oliva‐Teles, A., Heras, V., Mancera, J. M., Costas, B. (2015). Evaluation of different stocking densities in a Senegalese sole (Solea senegalensis) farm: Implications for growth, humoral immune parameters and oxidative status. Aquaculture, 438, 6–11. https://doi.org/10.1016/j. aquaculture.2014.12.034

Barton, B.A., 2002. Stress in fishes: a diversity of responses with particular reference to

changes in circulating corticosteroids. Integr. Comp. Biol. 42, 517–525

Caipang, C.M.A., Berg, I., Brinchmann, M.F. & Kiron, V. 2009. Short-term crowding stress in Atlantic cod Gadus morhua L. modulates the humoral immune response. Aquaculture, 295: 110-115.

Cemek, M., Büyükokuroğlu, M. E., Hazman, Ö., Konuk, M., Bulut, S., & Birdane, Y. O. (2011). The roles of melatonin and vitamin E plus selenium in prevention of oxidative stress induced by naloxone‐precipitated withdrawal in heroin‐addicted rats. Biological Trace Element Research, 142(1), 55–66. https://doi.org/10.1007/s12011-010-8744-8

Costas, B., Aragao, C., Dias, J., Afonso, A., & Conceição, L. E. C. (2013). Interactive effects of a high‐quality protein diet and high stocking density on the stress response and some innate immune parameters of Senegalese sole Solea senegalensis. Fish Physiology and Biochemistry, 39(5), 1141–1151. https://doi.org/10.1007/s10695-013-9770-1

Dai, L.; Li, J.; Peng, X.; Yang, Q.; Xu, Q.; Dou, Z.; Gao, H. (2023). Effects of Stocking Density on Rice Yield, Rice Quality and Ecological Environment in the Coculture of Rice and Aquatic (poultry) Animals. China Rice, 29, 55–59.

Davis, A.K., Maney, D.L. & Maerz, J.C. (2008). The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology, 22: 760-772.

Dehler, C. E., Secombes, C. J., & Martin, S. A. (2017). Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.). Aquaculture, 467, 149–157. https://doi.org/10.1016/ j. aquaculture.2016.07.017

Eissa, M. A., & Abdel-Rahman, A. M. (2020). Effect of stocking density on growth performance, feed utilization, and proximate analysis of Nile tilapia (Oreochromis niloticus). Egyptian Journal of Aquatic Biology and Fisheries, 24(6), 49-56.

Fadil, M. S., Syaifullah and Zakaria I. J. (2011) Study of several aspects of water chemistry physics and fish physiological aspects found in a rubber factory’s exhaust stream in the Batang Arau River. Thesis, Graduate Program Andalas University, Padang, Indonesia.

FAO. (2019). Cyprinus carpio. Cultured Aquatic Species Information Programme. Food and Agriculture Organization of the United Nations. http://www.fao.org/fishery/ culturedspecies/ Cyprinus_carpio/en

Ghozlan, A.; Gaber, M.M.; Zaki, M.A.; Nour, A. Effect of Stocking Density on Growth Performance, Production Trait, Food Utilization and Body Composition, of Meagre (Argyrosomus regius). World J. Eng. Technol. 2018, 6, 37–47.

Grand, T. C., & Dill, L. M. (1999). The effect of group size on the foraging behaviour of juvenile coho salmon: Reduction of predation risk or increased competition? Animal Behaviour, 58(2), 443–451. https://doi.org/10.1006/anbe.1999.117

Henrique, M.M.F; Gomes, E.F; Gouillou-Coustans, M.F; Oliva-Teles, A. and Davies, S.J. (1998). Influence of supplementation of practical diets with vitamin C on growth and response to hypoxic stress of seabream, Sparus aurata. Aquaculture, 161: 415–426

Jørgensen, E.H.; Christiansen, J.S.; Jobling, M. (1993). Effects of stocking density on food intake, growth performance and oxygen consumption in Arctic charr (Salbelinus alpinus). Aquaculture 110, 191–204.

Li, X., Wu, X., Zhou, Y., & Yu, H. (2019). Effect of stocking density on growth, antioxidant capacity, and intestinal morphology of juvenile yellow catfish Pelteobagrus fulvidraco. Aquaculture, 504, 329-335.

Li, H. W., & Brocksen, R. W. (1977). Approaches to the analysis of energetic costs of intraspecific competition for space by rainbow trout (Salmo gairdneri). Journal of Fish Biology, 11(4), 329–341. https://doi.org/10.1111/j.1095-8649.1977.tb04126.x.

Liu, B., Y. Liu, and G. Sun. (2017). Effects of stocking density on growth performance and welfare-related physiological parameters of Atlantic salmon Salmo salar L. in recirculating aquaculture system. Aquaculture Research 48 (5):2133–44. doi:10.1111/are.13050.

Liu, B.; Jia, R.; Zhao, K.; Wang, G.; Lei, J.; Huang, B. (2016a) Stocking density effects on growth and stress response of juvenile turbot (Scophthalmus maximus) reared in land-based recirculating aquaculture system. Acta Oceanol. Sin., 36, 31–38.

Liu, Q., Hou, Z., Wen, H., Li, J., He, F., Wang, J., Guan, B., and Wang, Q. (2016b) Effect of Stocking Density on Water Quality and (Growth, Body Composition and Plasma Cortisol Content) Performance of Pen-Reared Rainbow Trout (Oncorhynchus mykiss) J. Ocean Univ. China DOI 10.1007/s11802-016-2956-2. ISSN 1672-5182, 15 (4): 667-675

Liu, Y., Yao, Y., Li, H., Qiao, F., Wu, J., Du, Z., & Zhang, M. (2016c). Influence of endogenous and exogenous estrogenic endocrine on intestinal microbiota in zebrafish. PLoS ONE, 11(10), e163895. https://doi.org/10.1371/journal.pone.0163895

Liu, B., Jia R., Han C, Huang B., and Lei JL. (2016d): Effects of stocking density on antioxidant status, metabolism and immune response in juvenile turbot (Scophthalmus maximus). Comp Biochem Physiol C Toxicol Pharmacol. 190:1–8.

Ni, M., Wen H., Li J., Chi M., Bu Y., Ren Y., Zhang M., Song Z. and Ding H., (2014) The physiological performance and immune responses of juvenile Amur sturgeon (Acipenser schrenckii) to stocking density and hypoxia stress. Journal of Fish & Shellfish Immunology 36(2):325-335.

Nicula, M. (2004). Fiziologia organismelor acvatice. In I. Fiziologia peştilor (Ed.), Fish hematology and circulatory physiology (Ist edn). Timişoara, Romania: Mirton.

Nikinma, M., & Huestis, W. H. (1984). Adrenergic swelling of nucleated erythrocytes: Cellular mechanisms in a bird, domestic goose, and two teleosts, striped bass and rainbow trout. Journal of Experimental Biology, 113, 215–224.

Oliveira, E.G., Pinheiro, A.B., Oliveira, V.Q., Silva-Júnior, A.R.M., Moraes, M.G., Rocha, I.R.C.B., Sousa, R.R., Costa, F.H.F. (2012). Effects of stocking density on the performance of juvenile pirarucu (Arapaima gigas) in cages. Aquaculture 370-371, 96–101.

Oliveira, V.Q., Matos, A.R.B., Bezerra, T.A., Mesquita, P.E.C., Oliveira, V.Q., Moraes, A.M., Oliveira, E.G., Moraes, M.G., Sousa, R.R., Costa, F.H.F. (2013). Preliminary studies on the optimum feeding rate for pirarucu Arapaima gigas juveniles reared in floating cages. International Journal of Aquaculture 3, 147–151.

Oliveira, EG, Santos FJS, Oliveira VQ, Mesquista PEC, Moraes MG, Sousa RR, (2020). Influence of stocking density on water quality and growth performance in production of juvenile pirarucu, Arapaima gigas, in irrigation canals. Braz J Develop; 6(2): 8725- 8743. http://dx.doi.org/10.34117/bjdv6n2-255.

Olsen, R. E., Sundell, K., Mayhew, T. M., Myklebust, R., & Ringø, E. (2005). Acute stress alters intestinal function of rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture, 250(1–2), 480–495. https://doi.org/10.1016/j.aquaculture.2005.03.014

Paredes-López, D., Robles-Huaynate, R., Rebaza-Alfaro, C. Delgado-Ramírez, J, & Aldava-Pardave, U. 2021. Effect of stocking density of juvenile Arapaima gigas on rearing water quality hematological and biochemical profile, and productive performance. Latin American Journal of Aquatic Research, 49(2): 193-201. DOI: 10.3856/vol49-issue2-fulltext-2588.

Pinho, S., Brol, J., Jacques de Almeida, E., Lemos de Mello, G., Jeronimo, G.T. & Cohelo-Emerenciano, M.G. (2016). Effect of stocking density and vertical substrate addition on growth, performance, and health status of fat snook Centropomus parallelus. Aquaculture, 457: 73-78.

Sangiao Alvarellos, S., Guzmán, J. M., Láiz Carrión, R., Míguez, J. M., Martín Del Río, M. P., Mancera, J. M., & Soengas, J. L. (2005). Interactive effects of high stocking density and food deprivation on carbohydrate metabolism in several tissues of gilthead sea bream Sparus auratus. Journal of Experimental Zoology Part A: Ecological and Integrative. Physiology, 303(9), 761–775. https://doi.org/10.1002/jez.a.203

Seo J, and Park J. (2022). Does Stocking Density Affect Growth Performance and Hematological Parameters of Juvenile Olive Flounder Paralichthys olivaceus in a Recirculating Aquaculture System? Animals (Basel). 22;13(1):44. doi: 10.3390/ani13010044. PMID: 36611653; PMCID: PMC9817517.

Swain Himanshu S., Das Basanta K., Upadhyay Aurobinda, Ramteke Mitesh H., Kumar Vikas, Meena, Dharmendra K., Sarkar Uttam K., Chadha Narinder K. & Rawat2 Kiran D. Stocking density mediated stress modulates growth attributes in cage reared Labeo rohita (Hamilton) using multifarious biomarker approach. Scientific Reports. 12:9869. https://doi.org/10.1038/s41598-022-13570-x

Tort, L. (2011): Stress and immune modulation in fish. Dev Comp Immunol. 35(12):1366–75.

Wang, Y.-W., J. Zhu, X.-P. Ge, S.-M. Sun, Y.-L. Su, B. Li, Y.-R. Hou, and M.-C. Ren. (2018). Effects of stocking density on the growth performance, digestive enzyme activities, antioxidant resistance, and intestinal microflora of blunt snout bream (Megalobrama phala) juveniles. Aquaculture Research 50 (1):236–46. doi:10.1111/are.13889.

Wang, Y., P. Xu, Z. Nie, Q. Li, N. Shao, and G. Xu. 2019. Growth, digestive enzymes activities, serum biochemical parameters and antioxidant status of juvenile genetically improved farmed tilapia (Oreochromis niloticus) reared at different stocking densities in in-pond raceway recirculating culture system. Aquaculture Research 50 (4):1338–47. doi:10.1111/ are.14010.

Xing, J., Zhan, W., & Zhou, L. (2002). Endoenzymes associated with haemocyte types in the scallop (Chlamys farreri). Fish & Shellfish Immunology, 13(4), 271–278. https://doi.org/10. 1006/fsim.2001.0402

Zarkasi, K. Z., R. S. Taylor, G. C. Abell, M. L. Tamplin, B. D. Glencross, and Bowman, J. P. (2016). Atlantic salmon (Salmo salar L.) gastrointestinal microbial community dynamics in relation to digesta properties and diet. Microbial Ecology 71 (3):589–6. doi:10.1007/s00248- 015-0728-y.