Mariam Ali Kki Mohamed Ali
Department of Biotechnology, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamil Nadu, India
Prakash B
Department of Biotechnology, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamil Nadu, India
Ling Shing Wong
Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Nilai 71800, Negeri Sembilan, Malaysia
Kumar Krishnan
Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Nilai 71800, Negeri Sembilan, Malaysia
Durgadevi Ravindran
Department of Biotechnology, Vels Institute of Science Technology & Advanced Studies, Chennai, Tamilnadu, India.
Sowmiya Hari
Department of Bio Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamil Nadu, India
Ashok Kumar K
Department of Biotechnology, Vels Institute of Science Technology & Advanced Studies, Chennai, Tamilnadu, India.
Jayanthi M
Department of Biotechnology, Vels Institute of Science Technology & Advanced Studies, Chennai, Tamilnadu, India.
Suganthi M
Department of Biotechnology, Vels Institute of Science Technology & Advanced Studies, Chennai, Tamilnadu, India.
J.Manjunathan
Department of Biotechnology, Vels Institute of Science Technology & Advanced Studies, Chennai, Tamilnadu, India.
Abirami G
Department of Biotechnology, Vels Institute of Science Technology & Advanced Studies, Chennai, Tamilnadu, India.
Abstract
Vibriosis is a major problem in shrimp farm. Farmers indiscriminately use hormones, antibiotics, disinfectants and other chemicals in fish feed and culture water to protect their crops. E. coli and Aeromonas sp were highly predominant isolates. Herbs act as agents in aquaculture to control or reduce pathogen infections. The results of phytochemicals screening of fruit extract of Phyllanthus emblica showed the presence of various phytchemicals. Phyllanthus emblica extract shows anti-bacterial properties against biofilm producing shrimp pathogens. Among the 10 bacterial genera, E. coli and P.aeruginosa were highly suppressed. The extracts exhibited the bacteria growth inhibitory activity in a dose-dependent manner. The compound sitosterol has potent antibiofilm activity was showed by molecular docking, which revealed a significant binding energy and interaction (-8.4 Kcal/mol) between it and key biofilm-forming protein. Diving analysis was done using the chemical compounds found in GCMS analysis.
