Anticancer Potential Of Corchorus Olitorius L Leaves Aqueous Extract In Osteosarcoma
DOI:
https://doi.org/10.53555/jaz.v43iS1.5293Keywords:
Osteosarcoma, anticancer, Corchorus olitorius L leaves, MMP9, mRNA.Abstract
INTRODUCTION
Osteosarcoma is the most common histological form of primary bone sarcoma. It is most prevalent in teenagers and young adults. Osteosarcoma is the eighth-most common form of childhood cancer, comprising 2.4% of all malignancies in pediatric patients, and about 20% of all primary bone cancers. The treatment of osteosarcoma involves surgery, and chemotherapy followed by radiotherapy. The anticancer potential of Corchorus olitorius L leaves can be used as an adjunct therapy in osteosarcoma. Tumor cell growth and proliferation is regulated by MMPs and TIMPs .Matrix metalloproteinases (MMP-9) have been related to important roles in tumor invasion. Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) inhibits the activity of metalloproteinases
AIM
To analyze the anticancer potential of Corchorus olitorius L leaves aqueous extract in osteosarcoma.
OBJECTIVE
To study the effects of corchorus olitorius L leaves on the MMP 9 and TIMP 1 mRNA expression in the osteosarcoma cell line.
MATERIALS AND METHODS
Corchorus olitorius L leaves are collected and involved in the extraction process. The extraction process includes collection of leaves , grinding ,extraction using soxhelt apparatus, concentrating using Rotary evaporator.
After the process of extraction, osteosarcoma cells are cultured in the suitable medium, PH, temperature and ratio should be 95 to the carbon dioxide ratio of five.
Then the osteosarcoma cells are involved in treatment.
There are two treatments for osteosarcoma cells which are culture MTT assay and gene expression analysis. In gene expression analysis RNA isolation is done by harvesting the cells using RNA kit which contain Trizol reagent , chloroform, isopropanol and RNA pellet . After that it is converted to complementary DNA using real-time PCR which denotes the anti-cancer activity
RESULT
In the present study, Corchorus olitorius L leaves extract decreased MMP9 mRNA expression along with proliferation of osteosarcoma
Corchorus olitorius has contain flavanons such as quercetin and ascorbic acid
Many reports available on quercetin-induced anticancer activity in several cancers
CONCLUSION
In conclusion, the present study demonstrates for the first time corchorus olitorius decreased MMP 9 and TIMP 1 mRNA expression in osteosarcoma
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