Synthesis Of Substituted (4(1H-Indol-3-Yl)Butanoyl)-2-(1H-4-Indol-4-Ylidene)-N-(4hydroxybenzyl)Hydrazine-1-Carboxamide And It’s Anti-Inflammatory Studies

Abstract

Based on the provided context, a series of new indole-hydrazone derivatives were synthesized and their structures were determined through elemental and spectral studies. These synthesized compounds were then tested for their anti-inflammatory activity using carrageenan-induced oedema in albino rats, with indomethacin as the standard drug. The synthesized compounds exhibited a reduction in oedema formation ranging from 72.3% to 89.3%, surpassing the inhibition observed with indomethacin (46%). Additionally, the compounds were evaluated for their anticancer activity against the MCF-7 breast cancer cell line at a dose of 100µg/ml. Compounds 2f and 2j demonstrated mild anticancer activity with 61% and 68% inhibition, respectively, compared to doxorubicin. Furthermore, molecular docking studies were conducted using the Molego virtual docker program, which revealed that all prepared compounds exhibited high docking scores against both cox-1 and cox-2. The docking scores and binding energy correlated well with the pharmacological results. Lastly, the drug likeness and bioactivity of the compounds were predicted using Molinspiration software, indicating that all new compounds possess favorable drug likeness and bioactivity scores.