Comparative Evaluation of Bacterial Contamination and Antibacterial Efficiency in Charcoal Bristled Bamboo Tooth Brush and Charcoal Bristled Nylon Tooth Brush -An In vivo study

Abstract

Maintaining the oral hygiene reduces the load of microorganism and the oral diseases can be controlled. The use of charcoal bristled bamboo tooth brush has made a boon in oral hygienic practice owing its better cleaning efficiency Aim and Objective-The aim of this study was to compare and evaluate Bacterial Contamination and Antibacterial Efficiency in Charcoal bristled Bamboo Tooth Brush and charcoal bristled Nylon Tooth Brush. Materials and Method-A total of 50 patients met inclusion criteria were chosen for the study. Oral prophylaxis and oral hygienic instructions were given. All participants were given first with charcoal bristled nylon tooth brush and tooth paste. After a washout period of 14 weeks,it is replaced by charcoal bristled bamboo tooth brush. Bristles of both the tooth brushes sectioned separately and placed in a 5ml of saline, followed by isolating 0.1ml of saline and cultured in blood agar for 24hours of incubation. The colony forming units were counted later. To evaluate the antibacterial efficiency, the zone of inhibition was evaluated. Data collected were analysed and compared with student t-test.  Result-The mean colony forming units was found more in charcoal bristled bamboo tooth brush. The mean zone inhibition was found around 11.32mm for charcoal bristled nylon toothbrush and 4.94mm for charcoal bristled bamboo tooth brush. Conclusion-The study has shown the retention of colony forming units more in charcoal bristled bamboo tooth brush. The zone of inhibition found around charcoal bristle nylon tooth brush supported better antimicrobial properties compared to charcoal bristled bamboo tooth brush