SUBLETHAL EFFECTS OF IMIDACLOPRID ON HAEMATOLOGICAL AND BIOCHEMICAL PROFILE OF FRESHWATER FISH, CYPRINUS CARPIO
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Abstract
Imidacloprid is a commercialized Neonicotinoid pesticide, widely used to kill piercing and sucking types of insect pests in agriculture field. Due to its systemic nature and high water solubility, imidacloprid gradually reach and contaminate surrounding water bodies. The present study was planned to investigate the sublethal toxic impacts of pesticide imidacloprid on haematological and biochemical profile of freshwater fish, Cyprinus carpio. The fingerlings of Cyprinus carpio were exposed to three different sublethal concentrations of imidacloprid (7.8 ppm, 15.6 ppm and 23.4 ppm) for 30 days. Blood samples from all treatments were collected and used for hematological as well as biochemical diagnosis. During the trial period, haematological parameters like TEC, Hb and Hct were decreased significantly (P<0.05), whereas TLC count was found to be increased in imidacloprid treated fish. Similarly MCH count was lower but MCV was higher significantly as compared to control. In the biochemical analysis, plasma protein levels were significantly decreased in all the treatment groups when compared to control. Similar trends of significant decrease in the values of albumin and globulin was also reported. Thus, results of present study show that imidacloprid can cause remarkable alterations in the haematological and biochemical parameters of Cyprinus carpio.
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References
Oruc, E.O., 2010. Oxidative stress, steroid hormone concentrations and acetylcholinesterase activity in Oreochromis niloticus exposed to Chlorpyrifos. Pestic. Biochem. Physiol., 96:160-166. DOI: https://doi.org/10.1016/j.pestbp.2009.11.005
Adhikari, S., B. Sarkar, A. Chatterjee, C.T. Mahapatra & S. Ayyappan, 2004. Effects of cypermethrin and carbofuran haematological parameters and prediction of their recovery in a freshwater teleost, Labeo rohita (Hamilton). Ecotoxicol. Environ. Saf., 58: 220-226. DOI: https://doi.org/10.1016/j.ecoenv.2003.12.003
Sarkar, M., P. Biswas, S. Roy, R. Kole & A. Chowdhury, 1999. Effect of pH and type of formulation on the persistence of imidacloprid in water. B. Environ. Contam. DOI: https://doi.org/10.1007/s001289901023
Tox., 63: 604-609.
Hovda, L.R. & S.B. Hooser, 2002. Toxicology of newer pesticides for use in dogs and cats. Vet. Clin. Small. Anim., 32: 455-67. DOI: https://doi.org/10.1016/S0195-5616(01)00013-4
Krohn, J., 1989. Water solubility of NTN 33893. Miles report No. 99859. Bayer AG, Wuppertal-Elberfeld, Germany. 10.
Matsuda, K., S.D. Buckingham, D. Kleier, J. Rauh, M. Grauso & D.B, Sattelle, 2001. Neonicotinoids: insecticides acting on insect nicotinic acetylcholine receptors. Trends Pharmacol. Sci., 22: 73-80. DOI: https://doi.org/10.1016/S0165-6147(00)01820-4
Iwaya, K. & S. Kagabu, 1998. Biological properties of the chloronicotinyl insecticide imidacloprid: high selectivity and safer use in practice. Rev. Toxicol., 2: 121-132.
Tomizawa, M. & J.E. Casida, 2005. Neonicotinoid insecticide toxicology: Mechanisms of Selective Action. Annu. Rev. Pharmacol., 45: 247-68. DOI: https://doi.org/10.1146/annurev.pharmtox.45.120403.095930
Nemeth-Konda, L., G. Fuleky, G.Y. Morovjan & P. Csokan, 2002. Sorption behaviour of acetochlor, atrazine, carbendazim, diazinon, imidacloprid and isoproturon on Hungarian agricultural soil. Chemosphere, 48: 545-552. DOI: https://doi.org/10.1016/S0045-6535(02)00106-6
Halappa, R. & M. David, 2009. Behavioural responses of the freshwater fish Cyprinus carpio (Linnaeus) following sublethal exposure to chlorpyrifos. TrJFAS. 9: 233-238.
Ramanujan, S.M. & G. Mohanty, 1997. Thiodan induced sub lethal response on haematological parameters of H. fossils. J. Life Sci., 2: 8-13.
Jan, U., G.M. Shah & A.A. Bhat, 2012. HaematoBiochemical Parameters of Common Carp. Rep. Opinion, 4: 1-3.
Deka, C. & K. Dutta, 2012. Effects of Cypermethrin on Some Haematological Parameters in Heteropneustes fossilis (Bloch). The Bioscan, 7: 221-223.
Mulcahy, M.F., 1975. Fish blood changes associated with disease: a haematological study of pike lymphoma and salmon ulcerative dermal necrosis. In: W.E. Rebelin,
C.M. Madison, Ed.; The pathology of fishes. University of Wisconsin, USA, 925-944.
Dacie, J.V. & S.H. Lewis, 1963. Pratical Hematology. J & A Churchill Ltd, London. 16. Sahli, H., 1969. Lehrbuch der Klinischen Untersuchungs-Methoden, Leipsic, 5th Edn, 845.
Nelson, D.A. & M.W. Morris, 1989. Basic methodology: hematology and coagulation, part IV. In: D.A. Nelson, J.B. Henry, Ed.; Clinical Diagnosis, Management by
Laboratory Methods Seventeenth, Saunder Company, Philadelphia, USA, 578-625.
Lowry, O.H., N.J. Rosebrough, A.L. Farr & R.J. Randall, 1951. Protein measurement in the folin phenol reagent. J. Biol. Chem., 193: 265-275. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
Gordon, A.S., G.N. Goper & E.D. Zaryani, 1967. The Kidney and erythropoiesis. Sem. Haemat., 4: 337-343.
Nauen, R. & I. Denholm, 2005. Resistance of insect pests to Neonicotinoid insecticides: current status and future prospects. Arch. Insect. Biochem. Physiol., 58: 200-215. DOI: https://doi.org/10.1002/arch.20043
Cavas, T. & S. Ergene-Gozukara, 2005. Micronucleus test in fish cells: a bioassay for in situ monitoring of genotoxic pollution in the marine environment. Environ. Mol. Mutagen., 46: 64-70. DOI: https://doi.org/10.1002/em.20130
Lazzari, R., M.F. Duarte, V.M. Morsch, A.L. Pippi & V.P. Vierira, 2006. Effects of clomazone herbicide on hematological and some parameters of protein and carbohydrate metabolism of silver catfish Rhamdia quelen. Ecotoxicol. Environ. Saf., 65: 48-55. DOI: https://doi.org/10.1016/j.ecoenv.2005.06.008
Bhatnagar, A., N. Cheema & A.S. Yadav, 2017. Alterations in Haematological and Biochemical Profile of Freshwater Fish, Cirrhinus mrigala (Hamilton) Exposed to
Sub-lethal Concentrations of Chlorpyrifos. Nat. Env. & Poll. Tech., 16: 1189-1194.
Reddy, D.C., P. Vijayakumari, V. Kalarani & R.W. Davies, 1992. Changes in erythropoitic activity of Sarotherodon mossambicus exposed to sublethal concentrations of the herbicide diuron. B. Environ. Contam. Tox.,49: 730-737. DOI: https://doi.org/10.1007/BF00200787
Combs, G.F., 2008. The Vitamins: Fundamental Aspects in Nutrition and Health. Elsevier, San Diego, 95-143.
Eisler, R. & P.H. Edmunds, 1996. Effect of methyl parathion and methyl oxychlor on blood and tissue chemistry of a marine fish. A.F.S., 95: 153-159. DOI: https://doi.org/10.1577/1548-8659(1966)95[153:EOEOBA]2.0.CO;2
Benarjee, G. & T. Rajendranath, 1990. Hematological changes induced by an organophosphorus insecticide in a freshwater fish Clarias batrachus (Linnaeus). Trop.
Freshwat. Biol., 2: 197-202.
Tavares, D.M., M.L. Martins & K.S. Nascimento, 1999. Evaluation of the hematological parameters in Piaractus mesopotamicus Holmberg (Osteichthyes, characidae) with Argulus sp. (Crustacea, Branchiura) infestation and treatment with organophosphate. Rev. Bras. Zool., 16: 553- 555. DOI: https://doi.org/10.1590/S0101-81751999000200019
Ahmadivand, S., H. Farahmand, A.R. Mirvaghefi, S. Eagderi & H. RahmatiHolasoo, 2014. Histopathological and haematological response of male rainbow trout (Oncorhynchus mykiss) subjected to butachlor. Vet. Med., 59: 433-439. DOI: https://doi.org/10.17221/7683-VETMED
Reddy, P.M. & M. Bashamohideen, 1989. Fenvalerate and cypermethrin induced changes in the haematological parameters of Cyprinus carpio. Acta. Hydrochim. DOI: https://doi.org/10.1002/aheh.19890170116
Hydrobiol., 17: 101-107.
Ural, M.S., 2013. Chlorpyrifos induced changes in oxidant/antioxidant status and haematological parameters of Cyprinus carpio: Ameliorative effect of lycopene. DOI: https://doi.org/10.1016/j.chemosphere.2012.12.006
Chemosphere, 90: 2059-2064.
Strmer, J., E.B. Jensen & J.C. Rankin, 1996. Uptake of nitrite, nitrate and bromide in rainbow trout, Onchorhynchus mykiss: effects on ionic balance. Can. J. Fish Aquat. Sci., 53: 1943-1950. DOI: https://doi.org/10.1139/cjfas-53-9-1943
Jensen, F., 1990. Nitrite and red cell function in carp: control factors for nitrite entry, membrane potassium ion permeation, oxygen affinity and methaemoglobin formation. J. Exp. Biol., 152: 149-166. DOI: https://doi.org/10.1242/jeb.152.1.149
Vedel, N.E., B. Korsgaard & F.B. Jensen, 1998. Isolated and combined exposure to ammonia and nitrite in rainbow trout (Oncorhynchus mykiss): effects on electrolyte status, blood respiratory properties and brain glutamine/ glutamate concentrations. Aquat. Toxicol., 41: 325-342. DOI: https://doi.org/10.1016/S0166-445X(97)00071-4
Ismail, M., R. Ali & M. Shahid, et al., 2018. Genotoxic and hematological effects of chlorpyrifos exposure on freshwater fish Labeo rohita. Drug. Chem. Toxicol., 41: 22- 26. DOI: https://doi.org/10.1080/01480545.2017.1280047
Santhakumar, M., M. Balaji & K. Ramudu, 1999. Effect of sub-lethal concentration of monocrotophos on erythropoietic activity and certain haematological parameters of fish Anabas testudineus (Bloch). B. Environ. Contam. Tox., 63: 379-384. DOI: https://doi.org/10.1007/s001289900991
Siddique, M.S. & D.D. Wanule, 2010. Acetamiprid indused changes in total leucocytes and erythrocytes count in a fresh water fish Channa punctatus (Bloch). The
Bioscan., 5: 163-164.
Zubair, A., 2011. Acute toxicity and haematological changes in common carp (Cyprinus carpio) caused by diazinon exposure. Afr. J. Biotechnol., 10: 13852- DOI: https://doi.org/10.5897/AJB11.1247
Dhembare, A.J. & G.M. Pondha, 2000. Hematological changes in fish, Punctius sophore exposed to some insecticides. J. Exp. Zool. India., 3: 41-44.
Wright, H.P., 1960. Medical physiology and bio physics. In: T.C. Ruch and J.F. Fulton, 18th Ed.; Saunder’s Company, Philadelphia. 41. Kumar, R. & T.K. Banerjee, 2016. Arsenic induced hematological and biochemical responses in nutritionally important catfish Clarias batrachus (L.). Toxicol. Rep., 3: 148- 152. DOI: https://doi.org/10.1016/j.toxrep.2016.01.001
Sweety, R.R., M. Ramesh, K.S. Sajwan & S.K. Kumar, 2008. Influence of zinc on cadmium induced haematological and biochemical responses in a freshwater teleost fish Catla catla. Fish Physiol. Biochem., 34: 169-174. DOI: https://doi.org/10.1007/s10695-007-9157-2
Singh, D. & A. Singh, 2003. Effect of stem bark extract of some common plants on non-target fresh water fish Channa marulius (Ham.). Indian j. fish, 50: 525-531.
Radha, M.V., 2005. Immunological effect of cadmium in Heteropneustes fossilis Bloch. Glob. Vet., 4: 544-547.