Investigation of Protein Patterns by Using Sds Page in Kidney, Liver and Brain of Cyprinus Carpio

Abstract

Western transfer and dodecyl sodium sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) are two of the most widely used and beloved techniques in cancer research to identify proteins and glycoproteins. the protein fraction expression on the protein profile is significantly lower on the seventh day than it is on the eighth, the protein fraction expression is directly inversely related to the number of days. In order to determine the number of protein bands expressed in different tissues of Cyprinus carpio exposed to mercury and lead as the number of days rose, electrophoretic research was conducted. The difference between the 27 bands for lead and 36 bands for mercury on the seventh day and the 29 bands for lead and 40 bands on the thirty-first day suggests that as the number of exposure days to lead and mercury rises, so do the number of protein bands that are expressed. Using gel electrophoresis, a mixture of proteins is categorized by kind and molecular weight in the Western Blotting method. The results are then put into a membrane, which results in the formation of a band for each protein. Two HSP70 gene bands were found in Cyprinus carpio tissues after exposure to lead and mercury.