Protein Content of Sea Urchins (Tripneustes Gratilla) and The Potential of Its Shell as An Antibacterial
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Abstract
Sea urchins are often used as a food ingredient by taking their gonads. Sea urchins are also a potential fishery resource that greatly benefits human life. The study aimed to determine the gonad protein levels of sea urchins (Tripneustes gratilla) in different aquatic environments. The variables observed in this study were sea urchin gonadal index, gonadal index, protein content analysis, and antibacterial sea urchin shell methanol extract against E. coli, Salmonella sp., and S. aureus. The results showed that the average gonadal index for sea urchins (Tripneustes gratilla) on these two substrates was still relatively low, 1.46 for sandy substrates and 1.72 for rocky substrates. The gonad protein content of sea urchins can be seen on a sandy substrate and overgrown with seagrass. The average percentage of gonad protein content in sea urchins is 17.3%, while on a rocky substrate, the average percentage of protein content is 16.5%. The results of the T-test showed that the average results for the gonad protein content of sea urchins on these two substrates were not significantly different. Concluded that the gonad protein content of rocky sea urchins had a higher value of 17.3 with a gonadal index of 1.72 compared to the sandy substrate with a gonadal protein content of 16.5 with a gonadal index of 1.46. The highest content of essential amino acids in rocky locations was found in the type of amino acid leucine, with a concentration value of 8.59. Antibacterial methanol extract of Tripneustes gratilla sea urchin shell against E. coli, salmonella sp., and S. aureus showed the diameter of the inhibition zone formed on the agar media; in this case, the bacterial inactivation unit ranged from 1.84 mm/g – 2.65 mm/g.
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