Production and Optimization of Laccase from Daedaleopsis confragosa by using Agro-Residual Wastes
DOI:
https://doi.org/10.53555/jaz.v43i1.4944Keywords:
Daedaleopsis confragosa, Laccase, Solid State FermentationAbstract
A laccase producing fungus Daedaleopsis confragosa was obtained from tree trunk from Karwar coastal region of Karnataka, India. Daedaleopsis confragosa produces laccase by utilizing the agro-wastes. The study revealed that the rice bran was found to be the best supported lignocellulosic substrate for extracellular laccase production under Solid state fermentation and optimum condition for highest laccase activity of pH and temperature were observed at 6.0 and 45 ℃ respectively. Glucose and peptone were the best supported carbon and nitrogen source for the maximum laccase activity. The optimum inoculum size and incubation periods for laccase maximum activity at 6 mm of 5 fungal discs and 10th day, respectively. Solid-state fermentation (SSF) has emerged as a potential technology for the production of microbial products such as feed, fuel, food, industrial chemicals and pharmaceutical products. Its application in bioprocesses such as bioleaching, bio-beneficiation, bioremediation, bio-pulping, etc. has offered several advantages. Utilisation of agro-industrial residues as substrates in SSF processes provides an alternative avenue and value-addition to these otherwise under- or non-utilised residues. The objective of this research work was to determine the most promising fungus, the best solid substrate and the optimal conditions for the production of laccase.
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Copyright (c) 2022 Sudheer I. Manawadi, Sanjotha G, Muhammad Zafar Iqbal Navalgund, Adiveppa B. Vantamuri
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