Detection of Hepatitis B virus (HBV) by using the RT- PCR and Serological Assay for Detecting Surface Antigen HBsAg

Abstract

The diagnosis of hepatitis B virus (HBV) in Iraq depend on the clinical symptoms and the positively serological assay and within which direct examination of the surface antigen (HBsAg) in serum. CDC and WHO recently considered the detection viral DNA interaction by real time-polymerase chain reaction (RT-PCR) as a standards laboratory for the diagnosis of HBV, therefore the aim of study is to evaluate this method in the diagnosis of HBV and a feasibility of this test as compared with the traditional way serological assay for direct detection of surface antigen (HbsAg) and recognize the ability of each test to find a relationship between the infection frequency and  the gender to know the capacity of each scan in determining the extent of the disease . were taken samples from patients (33male and27female) that clinically 60 bloods suspected to be infected with HBV and compared the direct serological assay with RT-PCR ratios. The result showed that were 100%, 63.3% and chi square values ​​were 1.279 , 1.865  respectively, F-test showed significant difference among females 10.22  and male 9.36  when examined by RT - PCR , followed by test Duncan which determined the different viral loads in both sexes . Our findings showed that RT –PCR offers a way sensitive, efficient and accurate for the diagnosis and determination the extent of HBV infection.