Response surface methodology used evaluation of the combined influence of pH, sucrose and BAP on in vitro propagation of Momordica cochinchinensis (Lour.) Spreng. (Cucurbitaceae)
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Abstract
Momordica cochinchinensis (Lour.) Spreng. is a tropical dioecious plant belonging to the family, Cucurbitaceae also known as Gac, baby jack fruit, cochinchin gourd and sweet gourd. The plant is noted for its densely orange fruit, rich in nutrients, especially with 70 times more lycopene content than tomatoes. It possesses medicinal properties and the seeds are used in traditional Chinese medicine called Mubiezhi to treat fluxes, liver and spleen disorders, wounds and hemorrhoids. This study evaluated the effect of combination of pH, sucrose and BAP concentration on in vitro multiple shoot induction response of M. cochinchinensis according to guidelines of design of experiment. For those three factors, Response surface methodology (RSM) using the Box-Behnken design (BBD) was chosen and applied to the design matrix. The response shoot number/explant was evaluated by in vitro culture under variable conditions given in the design matrix. The response shoot number/explant was evaluated using in vitro culture under variable conditions given in the design matrix. The outcome of this method is an equation that calculates the shoot number as a function of pH, sucrose, and BAP concentration This study created response surface plots and contour plots for each factor, by analysing each factor's level of influence on the response shoot induction.
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