Combinde And Comparative Biochemical And Behavioral Assessment Of Delphinium Denudatum And Amaranthus Spinosus For Anti-Stress, Nootropic And Antioxidant Activities
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Abstract
Background: Stress defined as an imprecise reaction of the body to any kind of stimuli on it and disturbs normal physiological condition, threatened homeostasis. Stress causes decline in health by disturbing behaviour, physical and hormonal system of the body.
Methods: The test drugs Dephinium denudatum root and Amaranthus spinoses leaves were defatted with petroleum ether (60-80 0C) and then extracted with hydroalcohlic solvent (Ethanol 95%, v/v: water, 1:1) by soxhlation process. The hydroalcohlic extract of both the drugs singly and in combinations was evaluated for experimental activity in Wistar albino rats in the doses of 200 and 400 mg/kg by using different anti-stress tests like Swimming endurance and post swimming muscle coordination test (physical stress), Immobilization stress test and Anoxia stress tolerance test, antioxidant activity by DPPH, Reducing power methods, Nitric oxide scavenging activity, and H2O2 assay method and nootropic activity was done by Elevated plus maze test, Morris water maze test and estimation of Acetylcholine esterase level.
Results: In the dose dependent manner, both the hydroalcohlic extracts and combination of the higher doses produced the anti-stress activity, antioxidant and nootropic activities.
Conclusion: Hydroalcohlic extracts of Dephinium denudatum root, Amaranthus spinoses leaves and combination of both the drugs may act as anti-stress, antioxidant and nootropic agents in rats. Amaranthus spinoses was found to be more effective compare to the Dephinium denudatum.
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