GC-MS analysis and In-vitro anti-diabetic activity of ethanolic extract of theAtrocarpus Heterophylus (Unriped Jack Fruit)
Main Article Content
Abstract
Objective: In the present study the ethanolic extracts of Atrocarpus Heterophylus (ETAH) were studied for Aldose reductase, alpha (α)- amylase and alpha (α)-glucosidase inhibition using an in-vitro anti diabetic and evaluate the GC-MS analysis of active compounds of ETAH.
Methods: The serial extraction was carried out with a series of solvents: Petroleum ether, ethyl acetate and ethanol with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were subjected to the antidiabetic activity was assessed by employing standard in-vitro techniques.
Results and discussion: The result showed ethanolic extract exhibited significant aldose reductase, α-amylase and α-glucosidase inhibitory activities with an all plant extracts respectively and well compared with standard acarbose drug. GC-MS analysis results revealed that ETAH contain the compounds are Cyclohexanol and Nonadecene. This knowledge will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics.
Conclusion: The investigation confirms that ethanolic extract exhibited highest antidiabetic activity among all extracts, Additional studies on needed for purification, characterization and structural elucidation of bioactive compounds from ethanolic extract
Downloads
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Veeresham C, Swetha E, Rao AR, Asres K. In vitro and in vivo aldose reductase inhibitory activity of standardized extracts and the major constituent of Andrographis paniculata. PhytotherRes., 2013; 27(3), 412–416.
Spector A. Some aspects of Dr Kinoshita's contributions to lens protein chemistry. Exp Eye Res., 1990; 50(6), 689–694.
Pierre russo, In Surgical Pathology of the GI Tract, Liver, Biliary Tract, and Pancreas (Second Edition), 2009.
Donath MY, Ehses JA. Type I and type II diabetes: NOD the diabetes we thought it was. Proc Natl Acad Sci., 2006; 103, 12217-12218.
PappuSrinivasan1,Arumugam Sudha1, P.Bharathajothi, Palanivel Rameshthangam, Ramar Manikandan. Effects of anti-inflammatory and anti-pyretic activity of Anisomelesmalabarica., Journal of Pharmacy Research, 2010; 3 (7).
Hansotia T, Drucker DJ. GIP and GLP-1 as incretin hormones: lessons from single and double incretin receptor knockout mice. RegulPept., 2005; 128, 125-134.
Venkatesan N, Anton Smith A. Effect of active fraction isolated from the leaf extract of Leptadenia reticulata on plasma glucose concentration and lipid profile in streptozotocin-induced diabetic rats. Chin J Nat Med., 2014;12, 0455–0460.
Pepato MT, Mori DM, Baviera AM. Fruit of the jambolan tree (Eugenia jambolana) and experimental diabetes. J Ethnopharmacol, 2005; 96, 43 - 48.
Hayman S, Kinoshita JH. Isolation and Properties of Lens Aldose Reductase. J Biol Chem., 1965; 240(2), 877.
Ranilla, Lena Galvez, Kwon, Young-In, Genovese, Maria Ines, Lajolo, Franco Maria, Shetty, Kalidas. Antidiabetes and antihypertension potential of commonly consumed carbohydrate sweeteners using in vitro models. Journal of Medicinal Food., 2008; 11, 337– 348.
Venkatesan N, Anton Smith A. Effect of active fraction isolated from the leaf extract of Dregeavolubilis [Linn.] Benth. on plasma glucose concentration and lipid profile in streptozotocin-induced diabetic rats. SpringerPlus 2013, 2:394.
Vogel GH, Vogel WH. Drug Discovery and Evaluation -Pharmacological assay, Springer-Verlag: Germany., 1997, 588-589.
Kumar YR, Reddy AR, Eswaraiah S, Mukkanti K, Reddy MS, Suryanarayana M.V. Structural characterization of impurities in pioglitazone. Pharmazie., 2004; 59(11), 836–839.
Narkhede M. B, Ajimire P.V, Wagh A.E. In vitro antidiabetic activity of Caesalpinadigyna(R.) methanol root extract. Asi J Plant Sci and Research., 2011;12, 101-106.
Banerjee A, Maji B, Mukherjee S, Chaudhuri K, Seal T. In Vitro Antidiabetic and Anti-oxidant Activities of Methanol Extract of Tinospora Sinensis. J App Biol Biotech., 2017; 5 (03), 061-067.