The Effects Of Hydro- Ethanolic Extract Of Euphorbia Hirta Leaves Extract On Hemato-Biochemical Changes In Cigarette Smoke-Exposed Rats

Main Article Content

Swarna Sudha
R. Padmini

Abstract

Cigarette smoke is a source of free radicals that cause health problems throughout the world. The study aimed to determine the effects of hydro- ethanolic extract of Euphorbia hirta leaves extract (EHLE) on hemato-biochemical changes in cigarette smoke exposed (CSE) rats. The study using 24 male Wistar rats consisted of Control (not exposed to cigarette smoke and given distilled water), group II Diseased group (cigarette smoke and given distilled water), group III treated group (Cigarette smoke and Euphorbia hirta leaves extract 200 mg/kg BW) and group IV plant alone (Euphorbia hirta leaves extract 200 mg/kg BW alone). Exposure to cigarette smoke and administration of extracts was carried out for 12 weeks. End of the experimental period, the rats were euthanized and dissected to collect the blood for hemato-biochemical studies. The results of the study showed that treatment with Euphorbia hirta leaves extract to CSE rats restored the hematological parameters, liver and kidney markers.

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How to Cite
Swarna Sudha, & R. Padmini. (2024). The Effects Of Hydro- Ethanolic Extract Of Euphorbia Hirta Leaves Extract On Hemato-Biochemical Changes In Cigarette Smoke-Exposed Rats. Journal of Advanced Zoology, 45(1), 903–913. https://doi.org/10.53555/jaz.v45i1.3466
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Author Biographies

Swarna Sudha

Research scholar, Department of Biochemistry, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamilnadu-600117.

R. Padmini

Head of the Department, Department of Biochemistry, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamilnadu-600117.

References

Adebayo, J. O., Adesokan, A. A., Olatunji, L. A., Buoro, D. O., & Soladoye, A. O. (2005). Effect of ethanolic extract of Bougainvillea spectabilis leaves on haematological and serum lipid variables in rats. Biokemistri, 17(1), 45-50.

Alizadeh, J., Jaffarzadeh, Z., Angali, K. A., & Ahmadizadeh, M. (2020). Exposure of cigarette smoke aggravates noise induced kidney damage. Journal of Renal Injury Prevention, 10(2), e12-e12.

Alsalhen, K. S., & Abdalsalam, R. D. (2014). Effect of cigarette smoking on liver functions: a comparative study conducted among smokers and non-smokers male in El-beida City, Libya. International Current Pharmaceutical Journal, 3(7), 291-295.

Bain, B. J., Rothwell, M., Feher, M. D., Robinson, R., Brown, J., & Sever, P. S. (1992). Acute changes in haematological parameters on cessation of smoking. Journal of the Royal Society of Medicine, 85(2), 80-82.

Bonsnss, R. W., & Taussky, H. H. (1945). On the colorimetric determination of creatmine by the Jatfe reaction. Journal of biological chemistry, 158, 581-591.

Bunout, D. (1999). Nutritional and metabolic effects of alcoholism: their relationship with alcoholic liver disease. Nutrition, 15(7-8), 583-589.

Dacie, J. V., & Lewis S. M. (1968). Practical Hematology, 4th edition J and A, Churchill, UK. 37:3-6.

Dubey, S., & Mehta, S. (2014). Hepatoprotective activity of Euphorbia hirta Linn. Plant against carbon tetrachloride-induced hepatic injury in rats. Food Bio. Med. Sci, 1, 108-111.

Durazzo, A., Lucarini, M., Souto, E. B., Cicala, C., Caiazzo, E., Izzo, A. A., & Santini, A. (2019). Polyphenols: A concise overview on the chemistry, occurrence, and human health. Phytotherapy Research, 33(9), 2221-2243.

Epstein, M. (1997). Alcohol’s impact on kidney function. Alcohol health and research world, 21(1), 84.

Goel, K., Gorkhali, R., Pradhan, S., & Gupta, S. (2017). Impact of Smoking and Smoking Cessation on Periodontal Health: A Review. Journal of Nepalese Society of Periodontology and Oral Implantology, 1(2), pp.65-71.

Hasan, F., Khachatryan, L., & Lomnicki, S. (2020). Comparative studies of environmentally persistent free radicals on total particulate matter collected from electronic and tobacco cigarettes. Environmental science & technology, 54(9), 5710-5718.

Insaf, A., & Raju, P. N. (2019). Potential of pure phytoconstituents and herbs in protection of drug induced nephrotoxicity. Indian Journal of Pharmaceutical Education and Research, 53(3), 400-413.

Kamceva, G., Arsova-Sarafinovska, Z., Ruskovska, T., Zdravkovska, M., Kamceva-Panova, L., & Stikova, E. (2016). Cigarette smoking and oxidative stress in patients with coronary artery disease. Open access Macedonian journal of medical sciences, 4(4), 636.

King, R. P. N., & Kind, E. J. (1954). Determination of alkaline phosphatase activity by colorimetric method. J. Clin. Path. 7, 322.

Kristina, S. A., Endarti, D., Sendjaya, N., & Pramestuty, O. (2016). Estimating the burden of cancers attributable to smoking using disability adjusted life years in Indonesia. Asian Pacific Journal of Cancer Prevention, 17(3), 1577-1581.

Li, Z. H., Velisek, J., Grabic, R., Li, P., Kolarova, J., & Randak, T. (2011). Use of hematological and plasma biochemical parameters to assess the chronic effects of a fungicide propiconazole on a freshwater teleost. Chemosphere, 83(4), 572-578.

Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951). Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 193(1), 265-275.

Malloy, H. T., & Evelyn, K. A. (1937). The determination of bilirubin with the photoelectric colorimeter. Journal of Biological Chemistry, 119(2), 481-490.

Maruna, R. F. L., & Trinder, P. (1958). Determination of serum sodium by the magnesium uranyl acetate. Clin. Chem. Acta, 2(58), 585.

Messner, B., & Bernhard, D. (2014). Smoking and Cardiovascular Disease. Arteriosclerosis, Thrombosis, and Vascular Biology, 34, pp.509-15.

Naganna, K. L. (1999). “Plasma proteinseds,” in Textbook of Biochemistry and Human Biology, G. P. Tawlar, L. M. Srivastava, and K. D. Moudgils, Eds., pp. 172–180, Prentice-Hall, New Delhi, India.

Natelson, S. (1957). Micro-techniques of clinical chemistry for the routine laboratory. C.C.Thomas, Spring-Field, Illinois, p: 381.

Nwaogwugwu, J. C., Okereke, S. C., Nosiri, C. I., Egege, A. N., & Akatobi, K. U. (2020). Hematological changes and antidiabetic activities of Colocasia esculenta (L. schatt) stem tuber aqueous extract in alloxan induced diabetic rats. Ann Clin Lab Res, 8(2), 313.

Ochei, J., & Kolhatkar, A. (2000). Medical Laboratory Science, Theory and Practice, Tata McGraw-Hill Publishing Company Limited, New Delhi, P 276-287.

Prashant Tiwari., Kumar, K., Pandey, A. K., Pandey, A., & Sahu, P. K. (2011). Antihepatotoxic activity of Euphorbia hirta and by using the combination of Euphorbia hirta and Boerhaavia diffusa extracts on some experimental models of liver injury in rats. International Journal of Innovative Pharmaceutical Research, 2(2), 126-130.

Ramesh, T., Sureka, C., Bhuvana, S., & Begum, V. H. (2015). Brain oxidative damage restored by Sesbania grandiflora in cigarette smoke-exposed rats. Metabolic brain disease, 30, 959-968.

Reitman, S., & Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American journal of clinical pathology, 28(1), 56-63.

Rodkey, F. L. (1965). Direct spectrophotometric determination of albumin in human serum. Clinical Chemistry 11, pp478-9.

Saxena, S., & Shahani, L. (2017). Ameliorative effect of broccoli powder on renal toxicity in mice caused by continuous exposure to Escitalopram Antidepressant Drug. Int. Res. J. Pharm, 8(11).

Shakhanbeh, J. M. (2016). Effect of prenatal cigarette smoke exposure on hematological characteristics in adult rat offspring. Jordan J Biol Sci, 9(3), 179-183.

Singh, C. R., & Kathiresan, K. (2015). Effect of cigarette smoking on human health and promising remedy by mangroves. Asian Pacific journal of tropical biomedicine, 5(2), 162-167.

Suganya, S., Sophia, D., Raj, C. A., Rathi, M. A., Thirumoorthi, L., Meenakshi, P., & Gopalakrishnan, V. K. (2011). Amelioration of nitrobenzene-induced nephrotoxicity by the ethanol extract of the herb Euphorbia hirta. Pharmacognosy Research, 3(3), 201.

Sunil Kumar., Malhotra, R., & Kumar, D. (2010). Euphorbia hirta: Its chemistry, traditional and medicinal uses, and pharmacological activities. Pharmacognosy reviews, 4(7), 58.

Tietz, N. W. (1986). Fundamentals of Clinical Chemistry, WB Saunders, Philadelphia, Pa, USA.

Tresserra-Rimbau, A., Lamuela-Raventos, R. M., & Moreno, J. J. (2018). Polyphenols, food and pharma. Current knowledge and directions for future research. Biochemical Pharmacology, 156, 186-195.

Tsao, R. (2010). Chemistry and biochemistry of dietary polyphenols. Nutrients, 2(12), 1231-1246.

Verma, R. J., & Patel, C. S. (2015). Effect of smoking on Haematological parameters in Human Beings. Journal of Cell and Tissue Research, 5(1), 337.

Vermehren, M. F., Wiesmann, N., Deschner, J., Brieger, J., Al-Nawas, B., & Kämmerer, P. W. (2020). Comparative analysis of the impact of e-cigarette vapor and cigarette smoke on human gingival fibroblasts. Toxicology in Vitro, 69, 105005.

WHO (World Health Organization) (2017) Depression and other common mental disorders: global health estimates. World Health Organization. Report no (WHO/MSD/MER/2017.2).