Effect of Some Levels from Ziziphus Spina-christi Leaves and Choline on Acute Liver Disease in Rats

Abstract

The study aims to investigate the effects of diets containing two levels of Ziziphus Spina-christi leaves, choline, and their combinationson nutritional evaluation, some biochemical analysis, anda histopathological examination of the livers of rats suffering from acute liver disease. This study involved the use of forty-eight male albino rats. Rats in this study were divided into2 main groups, as follows:The 1st main group (n=6 rats) was fed a basal dietas a negative control group. The 2ndmain group (42 rats) wasinjected subcutaneously with CCl4 in paraffin oil to induce acute liver disease in rats. The second main group of rats was divided into seven subgroups, each consisting of six rats,as follows: Subgroup (1)was only fed a basal diet (B.D.) as a control positive group. Subgroups 2 and 3were given diets containing 1% and 2% choline chloride, respectively. Subgroups 4 and 5were given diets containing 5% and 7.5% Zizyphus Spina-christi, respectively.Subgroups 6 and 7 were fed diets containing the combination of choline chloride and Zizyphus Spina-christi (1% choline chloride and 5% Zizyphus Spina-christi) and (2% choline chloride and 7.5% Zizyphus Spina-christi), respectively. The results indicated that injected rats with CCl4 increased liver enzymes, including AST, ALT, and ALP, while decreasing feed intake, body weight gain%, serum protein, albumin, globulin, and antioxidant enzymes, including (catalase "CAT", superoxide dismutase ("SOD"), glutathione ("GSH"), and glutathione peroxidase ("GPx"), as compared to non-injected rats. Treating acute liver disease rats with diets containing the two levels of Ziziphus Spina-christi leaves, choline, and their combinations improved all of these parameters and the histopathological changes in the liver as compared to non-treated rats. Conclusion: Ziziphus Spina-christi leaves, choline, and their combinationscan be used to reduce the side effects of acute liver diseases.