DNA Barcoding Of Coral Reef Associated Fishes Collected From Cuddalore, Southeast Coast Of India
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Abstract
DNA barcoding is the significant species identification method, it helps in the construction of phylogenetic tree and avert mislabeling of species. DNA barcoding of some of the coral reef associated fishes from Indian southeast coast were very scarce. Hence, in the present study DNA barcoding of five species of coral reef associated fishes such as Lutjanus russellii, Siganus canaliculatus, Siganus javus, Acanthurus tristis and Trachinocephalus myops collected from Cuddalore, southeast coast of India were studied. The sequences were submitted to the GenBank and their accession numbers were obtained. The GC content in the sequence of COI genes were also calculated, the maximum GC content was found in Trachinocephalus myops (50.47%) and minimum was in Acanthurus tristis (45.33%). The average GC content was 47.64 ± 1.01%. Among the five species analyzed the sequence for Acanthurus tristis was not available in the NCBI database earlier, hence this sequence may the first molecular evidence for GenBank database.
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References
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