Unlocking the secrets of DNA - A study of Biochemical Techniques in Genetic Research

Abstract

The unravelling of DNA's secrets has been a cornerstone of modern genetic research. This review delves into the multitude of biochemical techniques that have paved the way for a comprehensive understanding of DNA's structure, function, and interactions. From the pioneering days of X-ray crystallography to the contemporary era of high-throughput sequencing, these techniques have revolutionized the field and empowered researchers to decode the genetic information that underpins life. This review highlights key methodologies such as polymerase chain reaction (PCR), gel electrophoresis, DNA sequencing, recombinant DNA technology, and CRISPR-Cas9 genome editing. Furthermore, it explores how these techniques have enabled breakthroughs in areas like personalized medicine, biotechnology, and evolutionary biology. As genetic research continues to evolve, a deep appreciation of these biochemical techniques is essential for navigating the intricacies of DNA and unlocking its enduring mysteries