Study The Genetic Diversity Of Honey Bee Species In Nashik District Of Maharashtra, India
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Abstract
This study was planned to estimate the genetic diversity of honeybee species in Nashik district. Because of climatic variation different crops are taken all over the district. In this study, genetic diversity between Apis dorsata, Apis cerana, Apis florea and Apis mellifera is estimated from the study area. For this, DNA barcoding of samples was performed. Sequences were uploaded to NCBI and accession id numbers were generated. Data was subjected to MEGA X software which was used for computing the evolutionary divergence, transition\transversion bias. Kimura 2 model is used for data analysis. Divergence between honeybee species in Nashik district is ranges from 0.00479 to 0.148774. Maximum divergence is observed in Apis dorsata and Apis florea i.e. 0.148774. Neighbor- joining phylogenetic tree revealed close relatedness between Apis dorsata - Apis mellifera and Apis cerana - Apis florea. Estimated evolutionary divergence in Triambakeshwar tehsil ranges from 0.102 to 0.130. Apis dorsata and Apis mellifera showing minimum divergence i. e. 0.102 and Apis dorsata and Apis florea show maximum divergence i.e. 0.130. From this we can conclude that Apis dorsata and Apis mellifera are closely related while Apis dorsata and Apis florea are distantly. From Malegaon tehsil, the maximum divergence is 0.149 and minimum is 0.106.
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