DNA barcoding and Phylogenetic analysis for bio-surveillance of insect fauna and their identification in Seri ecosystem

Abstract

Molecular Studies on insect fauna of Seri ecosystem is vital to develop new strategies for pest control in sericulture. The growth of Seri-industry and the quality of silk production are directly interlinked with the pests’ control as it damages both Mulberry and Silkworm crops. In the current study, 36 different Mulberry pests of orders Lepidoptera, Hemiptera, Coleoptera, Orthoptera, Diptera, Hymenoptera, Araneae, and Mantodea were collected and reared. The total genomic DNA was isolated from individual insect species. Partial cytochrome oxidase I gene was amplified and molecular signatures were created in the form of DNA barcodes for correct identification and authentication of insect fauna related to the Seri ecosystem. This study is focused on Insect Biodiversity conservation to evolve sustainable measures. Biodiversity conservation studies are essential in Agriculture and its allied areas for biological control of pests. This multi-functional research study contributes to the scientific understanding of molecular systematics with flawless identification of insect fauna and a novel way of pest control and crop protection strategy.