Isolation, Screening and Characterization of Exopolysaccharide Producing Pseudomonas Sp. from The Soil Sample

Abstract

Exopolysaccharides (EPS) are vital metabolites that specific environmental microorganisms produce and expel. The goal of the current investigation is to identify bacteria that produce exopolysaccharides in soil samples from the Buttenath Dam region, At. Chanai, Tq. Ambajogai, Dist. Beed situated in the Marathwada region of Maharashtra, India. Two methods, such as gravimetric analysis of exopolysaccharide dry weight and quantification as, instance, for total carbohydrate content using phenol sulphuric acid technique, were used to screen the EPS-producing capacities of the chosen isolates. Post preliminary screening by selecting thick, ropy-like colony formers on agar medium, the results showed that thirteen (13) distinct colonies were marked to manufacture EPS. Tentative identification of these isolates was done based on morphological and biochemical tests. Three (03) isolates, VJ001, VJ003 and VJ010, were identified through secondary screening as the most effective EPS producers (producing precipitates over 1.46 mg/L of total dry weight and 8). This was in comparison to other bacterial colonies that were also isolated. Out of these screened isolates, VJ003 was the isolate with the highest exopolysaccharide produced and this isolated bacterium was identified using 16S rRNA gene sequence and phylogeny study as Pseudomonas aeruginosa VJ003 with NCBI GeneBank accession no. OR196093. The Pseudomonas aeruginosa VJ003 strain can be used in biotechnological sectors because of the ability it has to create significant levels of exopolysaccharides, according to the results of the current study.