Genetic association of GPX-1 gene polymorphism (rs1050450) with susceptibility to Chronic periodontitis– a case control study in south Indian population.
DOI:
https://doi.org/10.53555/jaz.v43i1.5260Keywords:
Chronic periodontitis, Polymorphism, Glutathione peroxidase, Ancestral allele, Genotype, Oxidative stress.Abstract
AIM: The purpose of this pilot study was to determine the association between the GPX-1 gene polymorphism (rs1050450) with susceptibility to Chronic Perodontitis in the south indian population.
INTRODUCTION: Periodontal disease is a widespread, chronic multimicrobial immuno-inflammatory illness. An essential enzymatic antioxidant, glutathione peroxidase (GPX-1), guards periodontal tissues against oxidative stress. Since members of the glutathione peroxidase family catalyse the reduction of organic hydroperoxides and hydrogen peroxide (H202) by glutathione and so protect cells from oxidative damage, the protein produced by this gene is a member of that family. Therefore, the disease genes in complex disorders are viewed as moderating disease genes.
MATERIALS AND METHODS: The study was conducted in saveetha dental college, chennai.
50 wet samples were collected after receiving ethical clearance, with N=25 case and N=25 control groups. After genomic extraction of the samples, PCR-RFLP based genotyping assays were performed using the specific forward and reverse primers flanking the locus at (rs1050450). The genotype distribution and allele frequencies between the study groups were determined using chi-square test.
RESULTS: For departure from Hardy-Weinberg equilibrium (HWE), chi square with one degree of freedom. The genotype frequency of cases and controls do not differ significantly χ 2df (P =0.5688). No significant difference was observed between the case and control groups as (P<0.05).
CONCLUSION: From the study, it has been concluded that there is no significant association of GPX-1 gene polymorphism rs1050450 with susceptibility to chronic periodontitis. Since it's a pilot study the sample size was kept as N=25 in the case group and control group. Sample size should be increased to determine the actual association between the genetic marker(rs1050450) and the disease phenotype (CP).
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To cite these references:
1. Murthykumar K, Arjunkumar R, Jayaseelan VP. Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis. J Investig Clin Dent. 2019;10(4):e12440. doi:10.1111/jicd.12440
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