Morphological and Molecular Identification of Aedes Aegypti through Genetic Marker Mitochondrial Encoded Cytochrome C oxidase I (MT-COI) Gene in Pakistan
DOI:
https://doi.org/10.53555/jaz.v45i6.4923Keywords:
Aedes aegypti, Buner, cytochrome oxidase-I, MT-COI, mosquitos, genetic markerAbstract
Aedes aegypti are slender, fragile, long-legged mosquito, which are distributed worldwide. In general, it is a terrestrial and nocturnal species that act as pollinator for few plant species as well as it is a hematophagous insect and act as main vector for many arboviruses (dengue, yellow fever, etc.). In many cities of Pakistan, dengue fever is becoming more prevalent as an infectious disease. as there is currently no vaccine for the dengue virus and control of the disease depends on vector control. The present study aims to investigate morphological and molecular identification of Aedes aegypti in district Buner. A total of 365 samples of mosquitoes were randomly collected from six tehsils of the district Buner. Before examination, all the collected samples were preserved in labelled Eppendorf tubes containing silica gel and transported to Veterinary Research & Disease Investigation Center (VR&DIC), Balogram Swat, Khyber Pakhtunkhwa, Pakistan for further laboratory analysis. Morphological identification was performed with the help of stereomicroscope using taxonomical keys. To increase identification accuracy, each sample was reconfirmed by PCR using mitochondrial Cytochrome oxidase first gene (COI). The amplified PCR product was sequenced, and the obtained sequences were trimmed by Bio Edit and BLAST in NCBI. After BLAST, phylogenetic tree was constructed by using MEGA-X. In phylogenetic tree, the sequence (COI) genes of Aedes aegypti species as previously reported from Sri Lanka and India. Furthermore, the results of this study will generate basic information about mosquitos spp; and it will help to understand the epidemiology of Aedes aegypti disease in Khyber Pakhtunkhwa, especially in district Buner, Khyber Pakhtunkhwa, Pakistan.
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Al-Azab, A., Zaituon, A., Al-Ghamdi, K., Al-Galil, F., 2022. Surveillance of dengue fever vector aedes aegypti in different areas in jeddah city saudi arabia. Adv. Anim. Vet. Sci. 10(2): 348-353. https://doi.org/10.17582/journal.aavs/2022/10.2.348.353
Aziz, A.T., 2023. Distribution and mitochondrial co1-based genetic diversity of aedes aegypti l (culicidae: Diptera) in saudi arabia. Saudi J. Biol. Sci. 30(3). http://dx.doi.org/10.1016/j.sjbs.2023.103566
Batovska, J., Blacket, M.J., Brown, K., Lynch, S.E., 2016. Molecular identification of mosquitoes (diptera: Culicidae) in southeastern australia. Ecol. Evol. 6(9): 3001-3011. https://doi.org/10.1002/ece3.2095
Bibi, M., Zahoor, M.K., Zahoor, M.A., Hafiz, M.A., Majeed, H.N., Nasir, S., Rasool, B., 2015. Genetic analysis of mosquitoes from rural and urban areas of sialkot, pakistan. Int J Agric Biol. 17(4). https://doi.org/10.17957/IJAB/14.0027
Centers for Disease Control and Prevention, 2020: Partners Available at: https://www.cdc.gov/mosquitoes/about/what-is-a-mosquito.html (Accessed 28 Feb 2023).
Chan, A., Chiang, L.-P., Hapuarachchi, H.C., Tan, C.-H., Pang, S.-C., Lee, R., Lee, K.-S., Ng, L.-C., Lam-Phua, S.-G., 2014. DNA barcoding: Complementing morphological identification of mosquito species in singapore. Para. & vect. 7(1): 1-12. https://doi.org/10.1186/s13071-014-0569-4
Cywinska, A., Hunter F., Hebert, P.D., 2006. Identifying canadian mosquito species through DNA barcodes. Med. Vet. Entomol. 20(4): 413-424. https://doi.org/10.1111/j.1365-2915.2006.00653
Dengue and severe dengue. World Health Organization, 2029: Partners Available at: https://www.who.int/news-room/questions-and-answers/item/dengue-and-severe-dengue. (Accessed 24 Feb 2023).
Dhakane, R., Ahirrao, K., Shinde, A., Kotule, J., 2021. DNA-based identification and control of disease spreading mosquito species: A review. J. appl. biol. biotechnol. 9(1): 124-134. http://dx.doi.org/10.7324/jabb.2021.9116
El-Badry, A.A., Mouhanad, A., El-Sayed W.S., El-Beshbishy, H.A., 2016. Phylogenetic analysis of aedes aegypti based on mitochondrial ND4 gene sequences in almadinah, saudi arabia. Iran. J. Biotechnol. 14(2): 58. https://doi.org/10.15171/ijb.1329
Escobar, D., Ortiz, B., Urrutia, O., Fontecha, G., 2022. Genetic diversity among four populations of aedes aegypti (diptera: Culicidae) from honduras as revealed by mitochondrial DNA cytochrome oxidase i. Path. 11(6): 620. https://doi.org/10.3390/pathogens11060620
Galtier, N., Nabholz, B., Glémin, S., Hurst, G., 2009. Mitochondrial DNA as a marker of molecular diversity: A reappraisal. Mol. Ecol. 18(22): 4541-4550. http://dx.doi.org/10.1111/j.1365-294x.2009.04380.x
Gullan, P.J., Cranston, P.S., 2014: The insects: An outline of entomology. John Wiley & Sons: 5th edn. Oxford, UK.
Khan, J., Khan, I., Ijaz, A., Iqbal, A., Salman, M., 2017. The role of vertical transmission of dengue virus among field-captured aedes aegypti and aedes albopictus mosquitoes in peshawar, Khyber Pakhtunkhwa, Pakistan. Pak. J. Zool. 49(3). https://doi.org/10.17582/journal.pjz/2017.49.3.777.784
Knowlton, N., Weigt, L.A., 1998. New dates and new rates for divergence across the isthmus of panama. Proc. R. Soc. Biol. Sci. 265(1412): 2257-2263. http://dx.doi.org/10.1098/rspb.1998.0568
Kumar, N.P., Kalimuthu, M., Kumar, M.S., Govindrajan, R., Venkatesh, A., Paramasivan, R., Kumar, A., Gupta, B., 2022. Morphological and molecular characterization of aedes aegypti variant collected from tamil nadu, india. J. Vector Borne Dis. 59(1): 22-28. https://doi.org/10.4103/0972-9062.331413
Mosquito taxonomic inventory myspecies info. Mosquito Taxonomic Inventory, 2023: Partners Available at: https://mosquito-taxonomic-inventory.myspecies.info (Accessed 23 Feb 2023).
Pfeiler, E., Flores-López, C.A., Mada-Vélez, J.G., Escalante-Verdugo, J., Markow, T.A., 2013. Genetic diversity and population genetics of mosquitoes (diptera: Culicidae: Culex spp.) from the sonoran desert of north america. Sci. World J. 2013. https://doi.org/10.1155/2013/724609
Polat, F., Serkan, D., 2021. Phylogenetic analysis of mosquito (diptera: Culicidae) species with mitochondrial cytochrome oxidase subunit 1 gene distributed in kocaeli. Cela. B. Uni. J. Sci. 18(2): 193-202. http://dx.doi.org/10.18466/cbayarfbe.1007398
Salgueiro, P., Serrano, C., Gomes, B., Alves, J., Sousa, C.A., Abecasis, A, Pinto, J., 2019. Phylogeography and invasion history of aedes aegypti, the dengue and zika mosquito vector in cape verde islands (west africa). Evol. Appl. 12(9): 1797-1811. https://doi.org/10.1111/eva.12834
Tahir, H.M., Mehwish, Kanwal, N., Butt, A, Khan S.Y., Yaqub, A., 2016. Genetic diversity in cytochrome c oxidase i gene of anopheles mosquitoes. Mt. DNA P. A, 27(6): 4298-4301. https://doi.org/10.3109/19401736.2015.1082104
Wan, Q.H., Wu, H., Fujihara, T., Fang, S.G., 2004. Which genetic marker for which conservation genetics issue? Elec. 25(14): 2165-2176. http://dx.doi.org/10.1002/elps.200305922
Wang, G., Li, C., Guo, X., Xing, D., Dong, Y., Wang, Z., Zhang, Y., Liu, M., Zheng, Z., Zhang, H., Zhu, X., Wu, Z., & Zhao, T., 2012. Identifying the main mosquito species in china based on DNA barcoding. PloS ONE 7(10): Article e47051. https://doi.org/10.1371/journal.pone.0047051
Weeraratne, T.C., Surendran, S.N., Parakrama Karunaratne, S., 2018. DNA barcoding of morphologically characterized mosquitoes belonging to the subfamily culicinae from sri lanka. Para. & Vect., 11(1): 1-10. https://doi.org/10.1186/s13071-018-2810-z
What Purpose do Mosquitoes Serve? -The National Wildlife Federation Blog, 2020: Partners Available at: https://blog.nwf.org/2020/09/what-purpose-do-mosquitoes-serve/#:~:text=Mosquitoes%20are%20Pollinators&text=Just%20like%20bees%20or%20butterflies. (Accessed 28 Feb 2023).
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Copyright (c) 2023 Fida Muhammad, Asad Ullah, Tayyaba Ilyas, Tahira Tayyeb, Mansoor Ahmad, Zulfiqar Ali, Rafiq Ullah, Muhammad Owais Khan, Maiz ur Rahman, Imad Khan, Raheela Taj, Shumaila Gul, Shakirullah Khan, Bushra Bibi
This work is licensed under a Creative Commons Attribution 4.0 International License.
