Invitro Regeneration And GUS Expression Studies In Ground Nut (Arachis Hypogaea L.) Variety ICGV 15311.

Swapna Mittapalli; Thriveni Dumpeti; Gade Pavan Kumar; Vaishnavi Anumula; Prathap Pasula; Suman Kalyan Sadhu; Rajender Vadluri

doi:10.53555/jaz.v44iS5.3151

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Published: Dec 31, 2023

DOI: https://doi.org/10.53555/jaz.v44iS5.3151

Keywords:

Agrobacterium Transformation, GUS expression, Ground nut, Embryo axis, Cotyledonary node, Leaf, Shooting, Rooting

Swapna Mittapalli

Thriveni Dumpeti

Gade Pavan Kumar

Vaishnavi Anumula

Prathap Pasula

Suman Kalyan Sadhu

Rajender Vadluri

Abstract

Ground nut is an important source of food,edible oil,dietary vitamin, minerals, proteins, animal feed etc. In this research, an efficient of regeneration and Agrobacterium mediated genetic transformation with GUS expression is reported in a commercially important variety ICGV 15311, using embryo axis, Leaf, Half cut embryo axis, Cotyledonary node. Various combinations and concentrations of plant growth regulators are used to obtain multiple shooting and rooting.MS (Murashig and Skoog) medium supplemented with various concentrations of Auxins (IBA,NAA,IAA and 2,4D) and Cytokinins (BAP and KIN).Explants cultured on 2,4 D and KIN expressed only swelling and enlargement without further development.BAP and NAA showed more shoots on embryo axis explants on medium concentration and combination of BAP (2.0 mg/l) and NAA(0.5mg/l).Rooting obtained on IBA (2.0mg/l). The bacterial culture 1.0 OD 600, incubation with 50 µl acetosyringone and co cultivation period of 3 days were found to be efficient for transformation. Tansformed putatives grown on shooting and rooting medium and remained healthy putatives transferred to soil for hardening.

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Swapna Mittapalli, Thriveni Dumpeti, Gade Pavan Kumar, Vaishnavi Anumula, Prathap Pasula, Suman Kalyan Sadhu, & Rajender Vadluri. (2023). Invitro Regeneration And GUS Expression Studies In Ground Nut (Arachis Hypogaea L.) Variety ICGV 15311. Journal of Advanced Zoology, 44(S5), 2405–2411. https://doi.org/10.53555/jaz.v44iS5.3151

Issue

Vol. 44 No. S5 (2023)

Section

Articles

This work is licensed under a Creative Commons Attribution 4.0 International License.

Author Biographies

Swapna Mittapalli

Department of Biotechnology, Chaitanya Deemed to be University. Kishanpura, Hanamkonda. 506009.

Thriveni Dumpeti

Department of Biotechnology, Chaitanya Deemed to be University. Kishanpura, Hanamkonda. 506009

Gade Pavan Kumar

Department of Biotechnology, Chaitanya Deemed to be University. Kishanpura, Hanamkonda. 506009

Vaishnavi Anumula

Department of Biotechnology, Kakatiya University, Hanamkonda. 506009.

Prathap Pasula

Department of Botany, RD Degree and PG college, Kishanpura, Hanamkonda. 506009

Suman Kalyan Sadhu

Department of Microbiology, Kakatiya University, Hanamkonda. 506009.

Rajender Vadluri

Department of Biotechnology, Chaitanya Deemed to be University. Kishanpura, Hanamkonda. 506009.

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