Agrobacterium Mediated Genetic Transformation Of Ground Nut (Arachis Hypogaea L.) Cultivar Icgv-15311 Embryo Axis Explants For Defensin Gene Against Fungal Resistance
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Abstract
Genetic transformation of Ground nut (Arachis hypogaea L.) was attempted using plasmid vector pCAMBIA '3365' Present with in Agrobacterium tumifaciens strain LBA4404 with CaMV35sp (Cauliflower mosaic virus 35s RNA Promoter). A total of 323 Embryo axis, Half cut embryo, Cotyledonary nodes were employed in independent co cultivation events, of which 13 putative transgenic plants were established in Invitro conditions. The transgenic shoots obtained were initially confirmed for the introduced reporter gene by GUS histochemical assay and later by the PCR analysis. The transformed shoots were checked for the transcription of the gene by RT-PCR analysis. Southern blot analysis was carried out to confirm the stable integration of the foreign DNA
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