Isolation And Identification Of Bacteria From Solid Waste Deposit Sites For The Degradation Of Pollutants
DOI:
https://doi.org/10.53555/jaz.v44iS7.2862Keywords:
Solid waste, disposal facility, Bacteria pollutants, Molecular techniques, BiodegradationAbstract
In and around the Treatment Storage and Disposal Facility (TSDF), Duindigal, Ramky site, Hyderabad, Telangana, ten samples were collected. To assess the ability of the bacterial isolates to remove contaminants, the following parameters were chosen for this study: pH, TDS, BOD, COD, TN, and TP. Two of the ten isolates showed promising degradation capabilities. The 16S rRNA gene sequence was used to apply genotypic techniques to the two strains that performed the best. Alcaligenes faecalis and Brevibacterium iodinum are the two bacterial strains that we found using NCBI BLAST analysis. To optimize the growth rate of the isolates, some parameters like pH, temperature, incubation time and peptone concentrations were optimized and got enhanced results. These findings have expanded the possibility of identifying industrially significant bacteria from solid waste disposal sites, and these isolates may be an essential source for the identification of enzymes with practical applications in industry.
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References
American Public Health Association, American Water Works Association, Water Environment Federation. Lipps WC, Braun-Howland EB, Baxter TE, eds. Standard Methods for the Examination of Water and Wastewater. 24th ed. Washington DC: APHA Press; 2023.
Caulier, S.; Nannan, C.; Gillis, A.; Licciardi, F.; Bragard, C.; Mahillon, J. Overview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group. Front. Microbiol. 2019, 10, 302.
Cooper KE. Theory of antibiotic inhibition zones in agar media. Nature. 1955;176(4480):510‒511.
Davami F, Eghbalpour F, Nematollahi L, Barkhordari F, Mahboudi F. Effects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG44 Cells; a New Insight into Amino Acid Profiles. Iran Biomed J. 2015;19(4):194-205. doi: 10.7508/ibj.2015.04.002. Epub 2015 Aug 1. PMID: 26232332; PMCID: PMC4649854.
Gupta, P., Samant, K., Sahu, A. (2012). Isolation of cellulose degrading bacteria and determination of their cellulolytic potential. International Journal Microbiology, 2012: 1-5.
Haq R, Zaidi SK, Shakoori AR. Cadmium resistant Enterobacter clacae and Klebsiella sp isolated from industrial effluents and their possible role in cadmium detoxification. World Journal of Microbiology and biotechnology. 1999;15(2):283‒290. https://doi.org/10.3390/microorganisms9061156.
Khaled Abuelhaded., et al. “Optimization of the Growth Condition and Molecular Identification of Two Bacterial Isolates from Egyptian Agricultural Wastes". Acta Scientific Microbiology 5.9 (2022): 73-79.
Kim, A.-L.; Park, S.; Hong, Y.-K.; Shin, J.-H.; Joo, S.-H. Isolation and Characterization of Beneficial Bacteria from Food ProcessWastes. Microorganisms 2021, 9, 1156.
Nakano MM, Zuber P. Molecular biology of antibiotic production in Bacillus. Crit Rev Biotechnol. 1990;10(3):223‒240.
Pacarynuk LA, Danyk HC. Biochemical Tests. In: Principles of Microbiology, Laboratory Manual. USA: Spring, TX; 2004:28‒34.
Patel J. B. (2001).16S rRNA gene sequencing for bacteria pathogen identification in the clinical laboratory. Molecular Diagnostics, 6(4): 313-332.
R. Wang, X. Hu, A.K. Agyekumwaa, X. Li, X. Xiao, Y. Yu Synergistic effect of kojic acid and tea polyphenols on bacterial inhibition and quality maintenance of refrigerated sea bass (Lateolabrax japonicus) fillets LWT-Food Science and Technology, 137 (2021), p. 110452, 10.1016/ j.lwt. 2020.110452.
Rastogi, M.; Nandal, M.; Khosla, B. Microbes as vital additives for solid waste composting. Heliyon 2020, 6, e03343.
Saha A, Santra SC. Isolation and characterization of bacteria isolated from municipal solid waste for production of industrial enzymes and waste degradation. J Microbiol Exp. 2014;1(1):12‒19. DOI: 10.15406/jmen.2014.01.00003.
X.-h. Sun, L.-r. Hao, Q.-c. Xie, W.-q. Lan, Y. Zhao, Y.-j. Pan, et al. antimicrobial effects and membrane damage mechanism of blueberry (Vaccinium corymbosum L.) extract against Vibrio parahaemolyticus Food Control, 111 (2020), p. 107020, 10.1016/j.foodcont.2019.107020.
Zhu, Z.; Bai, Y.; Lv, M.; Tian, G.; Zhang, X.; Li, L.; Jiang, Y.; Ge, S. Soil Fertility, Microbial Biomass, and Microbial Functional Diversity Responses to Four Years Fertilization in an Apple Orchard in North China. Hortic. Plant J. 2020, 6, 223–230.
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Copyright (c) 2023 Shaik Abdul Gafoor, Pandu. Brahmaji Rao, T. Raghavendra
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