Anti Urolithiatic Activity of Dolichos biflorus by Using Wistar Albino Rats in Ethylene Glycol Induction Method
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Abstract
Objective: Dolichos biflorus has been used in the Indian indigenous system of medicine, Ayurveda, for the treatment of various health ailments including renal calculi. The present study was undertaken to investigate the Anti-urolithic activity of ethanolic extract of Dolichos biflorus in Wistar Albino Rats.
Method: Dolichos biflorus extract was prepared by using 98% ethanol by maceration method and phytochemical analysis was performed. The samples were examined for antiurolithiatic action by determining phosphate, calcium, and oxalate levels in urine; Urea, uric acid, and creatinine in serum with the Automatic Laboratory Chemistry Research System. The histopathology of the kidney was analyzed.
Result: Dolichos biflorus extract at a dosage of 200mg/kg, 400mg/kg in Wistar Albino Rats Significantly, decreased the level of phosphate, calcium as well as oxalate in urine; decreased the serum level of urea nitrogen and creatinine, and uric acid. Comparing the observed outcomes at various dosages to the control groups revealed significant differences. The tubules exhibit normal morphology and have recovered to some extent. These results revealed that the seed extract of Dolichos biflorus decreased calculi levels.
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References
Samantha Ranaweera, Shanika Weerasinghe, Fatema Tarannum and Keisha. Inhibition and dissolution of calcium oxalate crystals and kidney stones by the extract of Kalanchoe pinnata. Journal of Medicinal Plants Research Vol. 17(6), pp. 201-217, June, 2023
Ratkalkar and Kleinman. Herbal plants used in the treatment of many biological process, Journal of Medicinal Plants Research, 2011;15(4):172-177
Bahmani et al., Study of stone according of their size and position, Journal of Medicinal Plants Research. 2016;15(4): 172-177
Atodariya U, Barad R, Upadhyay S. Anti-urolithiatic activity of Dolichos biflorus Seeds. J Pharmacognosy & Phytochem 2013;2:209-13.
Azwanida NNet al .A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 2015; 4:196.
Makasana A, Ranpariya V, Desai D, Mendpara J. Evaluation for the anti-urolithiatic activity of Launaea procumbens against ethylene glycol-induced renal calculi in rats. Toxicol Rep 2014; 1:46-52. Kaushik S, Choudhary Ml. Antiurolithiatic efficacy of combination preparations
Dolichos biflorus and Crata evanurvala: Folk medicines used in Indian traditional medicine. Future J Pharm Sci 2021; 4:12-8
Tyagi S, Patel C, Patel J. Review on Kidney Stones. Journal of Biomedical and Pharmaceutical Research 2012; 1(3):06-09.
Havagiray R, Chitme SA, Jain SK. Herbal treatment for urinary stones. IJPSR 2010; 1:1-10.
Kalpana Devi V, Baskar R. Biochemical effects in normal and stone forming rats treated with the ripe kernel juice of Plantain (Musa Paradisiaca). Ancient Science of Life 1993; 3&4:451-461.
Romero V, Akpinar H. Kidney stones: A global picture of prevalence, incidence, and associated risk factors, Rev Urol, 2010, 12(2-3), e86–e96.
Ghodasara J, Pawar A, Deshmukh C. Inhibitory effect of rutin and curcumin on experimentally-induced calcium oxalate urolithiasis in rats, Pharmacognosy Res, 2010, 2(6): 388– 392.
Cloutier J, Villa L, Traxer O. Kidney stone analysis: "Give me your stone, I will tell you who you are!", World J Urol, 2015, 33(2): 157–169.
Sarmistha Saha, P S Shrivastav & R J Verma. The antioxidative mechanism involved in the preventive efficacy of Bergenia ciliata rhizomes against experimental nephrolithiasis in rats. Pharmaceutical Biology 2014; 52(6): 712-722.
Azhar Shuaib Batoo, S Dey. Manzoor Ahmad Bhat, Mir Mudasir, Burhan Nabi. Acute toxicity 2018;7(6): 1229-123.
Mohibul Haque, Ali Salman Al-Shami. Asian Journal of pharmaceutical and clinical Research 2022;10.221-39.
Yasir Fet al., Effect of indigenous plant extracts on calcium oxalate crystallization having a role in urolithiasis. Urol Res. 2011; 39:345-350
Verley H. Practical Clinical Biochemistry. New Delhi: CBS Publishers; 2003:356.
Pandhare RB, Shende RR, Avhad MS, Deshmukh VK, Mohite PB, Sangameswaran B. Anti-urolithiatic activity of Bryophyllum pinnatum Lam. Hydroalcoholic extract in sodium oxalate-induced urolithiasis in rats. J Tradit Complement Med 2021; 11:545-51.
Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949; 177:751-766.
Anbu J, Suman S, Swaroop Kumar SL, Satheeshkumar. Antiurolithiatic Activity of Ethyl Acetate Root Extract of Ichnocarpus frutescens using Ethylene Glycol Induced Method in Rats. J Pharm Sci Res. 3; 2011: 1182–9.
Atmani F, Slimani Y, Minouni M. Prophylaxis of calcium stone by Hibiscus sabdariffa on experimentally induced nephrolithiasis in rats. BJU Int. 92; 2003:137–43.
Atef M,Al-Attar. Antilithiatic influence of spirulina on ethylene glycol-induced nephrolithiasis in male rats. Am J Biochem Biotechnol. 6; 2010:25–6.
Gilhotra U,Christina A. Effect of Rotula aquatica Lour. on ethylene-glycol-induced urolithiasis in rats. Int. J. Drug Dev. and Res. 3 (1); 2011: 273-280.
Pandhare RB, Shende RR, Avhad MS, Deshmukh VK, Mohite PB, Sangameswaran B. Anti-urolithiatic activity of Bryophyllum pinnatum Lam Hydroalcoholic extract in sodium oxalate-induced urolithiasis in rats. J Tradit Complement Med 2021; 11:545-51